Registration Dossier
Registration Dossier
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EC number: 201-855-4 | CAS number: 88-74-4
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: No GLP status given, test performed according to guideline.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Danish EPA Daphnid acute toxicity test, 40 CFR Ch. 1 (7-1-92 Edition), § 797.1300
- GLP compliance:
- no
Test material
- Reference substance name:
- 2-nitroaniline
- EC Number:
- 201-855-4
- EC Name:
- 2-nitroaniline
- Cas Number:
- 88-74-4
- Molecular formula:
- C6H6N2O2
- IUPAC Name:
- 2-nitroaniline
- Details on test material:
- - Name of test material (as cited in study report): 2-nitrobenzeneamine
- Analytical purity: 98%
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: isolated from Lake Langedam, Birkerod in 1979 and has been cultured at VKI since then.
- Age at study initiation (mean and range, SD): <24h
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- pH:
- 7.6-7.9
- Dissolved oxygen:
- 96-99 % saturation
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: all glass vessels 250 ml nominal volume, 125 tes tvolume
- Renewal rate of test solution (frequency/flow rate): once after 24 hours
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
- test medium: ISO standard medium - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 10.1 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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