Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: No GLP status given, test performed according to guideline.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: Danish EPA Daphnid acute toxicity test, 40 CFR Ch. 1 (7-1-92 Edition), § 797.1300
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 2-nitrobenzeneamine
- Analytical purity: 98%

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Source: isolated from Lake Langedam, Birkerod in 1979 and has been cultured at VKI since then.
- Age at study initiation (mean and range, SD): <24h

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

pH:
7.6-7.9
Dissolved oxygen:
96-99 % saturation
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: all glass vessels 250 ml nominal volume, 125 tes tvolume
- Renewal rate of test solution (frequency/flow rate): once after 24 hours
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6

- test medium: ISO standard medium
Reference substance (positive control):
yes
Remarks:
Potassium dichromate

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
10.1 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes