Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: No GLP status given, test performed according to guideline.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Danish EPA Daphnid acute toxicity test, 40 CFR Ch. 1 (7-1-92 Edition), § 797.1300
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2-nitroaniline
EC Number:
201-855-4
EC Name:
2-nitroaniline
Cas Number:
88-74-4
Molecular formula:
C6H6N2O2
IUPAC Name:
2-nitroaniline
Details on test material:
- Name of test material (as cited in study report): 2-nitrobenzeneamine
- Analytical purity: 98%

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Source: isolated from Lake Langedam, Birkerod in 1979 and has been cultured at VKI since then.
- Age at study initiation (mean and range, SD): <24h

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

pH:
7.6-7.9
Dissolved oxygen:
96-99 % saturation
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: all glass vessels 250 ml nominal volume, 125 tes tvolume
- Renewal rate of test solution (frequency/flow rate): once after 24 hours
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6

- test medium: ISO standard medium
Reference substance (positive control):
yes
Remarks:
Potassium dichromate

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
10.1 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes