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EC number: 202-269-1 | CAS number: 93-70-9
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1988-10-24 to 1988-11-18
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted and reported according to the OECD Guideline for the testing of chemicals 203 and principles of GLP.
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2'-chloroacetoacetanilide
- EC Number:
- 202-269-1
- EC Name:
- 2'-chloroacetoacetanilide
- Cas Number:
- 93-70-9
- Molecular formula:
- C10H10ClNO2
- IUPAC Name:
- 2'-chloroacetoacetanilide
- Details on test material:
- Molecular formula: C10 H10 Cl N 2
Storage: in the dark at 20 °C, under a hood
Appearance: crystalline, white powder
Molecular weight: 211,6 g/mol
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable.
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- Not applicable.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- Tween 80 was used as solubiliser.
The test substance was made into a paste using Tween 80 as solubilizing agent. Water for dilution was added and
the mixture homogenized using an Ultra-Turrax and ultrasonic bath.
The concentrations tested:
10, 35, 50, 71 and 100 mg/L
0 mg/L (negative control)
0 mg/L with 0.1 mL Tween 80 (control group)
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Source: West Aquarium, 3422 Bad Lauterberg
Species: Brachydanio rerio (Hamilton-Buchanan)
Acclimation period: at least 14 days before start of study in water for dilution.
Body length (measured from 10 representative fish): 2.4 - 3.3 cm, mean: 2.9, s: 0.22
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- No data available.
Test conditions
- Hardness:
- Reconstituted water, composition according to ISO/DIS 7346/1
- Test temperature:
- Concentration group: 21.6 - 23 °C
Control: 21.3 - 23 °C - pH:
- Concentration group: 7.2 - 8.2
Control: 7.5 - 8.3 - Dissolved oxygen:
- Concentration group: 2.3 - 9.2 mg/L
Control: 6.6 - 9.4 mg/L - Salinity:
- Not applicable.
- Nominal and measured concentrations:
- No data available.
- Details on test conditions:
- Static test system.
Test chambers: Glass, 10 l calibrated testing tank, (L 30 cm, W 22 cm, H 24 cm). The test chamber stood in a water bath made out
of Hostalit ZR with plexiglass viewing panel. The water bath was adjusted with a thermostat to 22°C.
Illumination: Day and Night frequency, each with 12 hours ., approx. 700 lux over the chamber. During the trial period the test champer was not
ventilated besides testing group with 71 mg/L of the 4. test i.e. after experiment start, from 48 hours, the oxygen level from at least 60% of the
saturation value could not more warranted. Therefore the testing chamber was ventilated by a glas capillary tube with a bubble frequency of 1-3 sec. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- LC0
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 50 - 71 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC100
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 50 - 71 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- Behavioural abnormalities: swimming and respiration abnormal
Necropsy: dead fish showed redded opercula; red and light coloured integument - Results with reference substance (positive control):
- No data available.
- Reported statistics and error estimates:
- No data available.
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Concentration Lethality
(mg/L) 48 h 96 h
absolute relative (%) absolute relative (%)
0 0/40 0 0/40 0
0* 0/10 0 0/10 0
10 0/10 0 0/10 0
35 0/10 0 0/10 0
50 0/10 0 0/10 0
71 9/10 90 9/10 90
100 10/10 100 10/10 100
LCO (mg/L)
highest measured 50 50
concentration whithout
Lethality
----------------------------------------------------------------------------------------------------------
LC50 (mg/L) 50 - 71 50 - 71
----------------------------------------------------------------------------------------------------------
LC100 (mg/L)
lowest measured 100 100
concentration with
100% Lethality
----------------------------------------------------------------------------------------------------------
* Solvent control with 0.1 mL Tween 80/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- LC50 (96 hrs) = 50 - 71 mg/L
- Executive summary:
The study was conducted and reported 1988 according to the OECD Guideline for the testing of chemicals 203 and principles of GLP.
The test item has a LC50 between 50 and 71 mg/L under the test condions (zebra fish, 96 hours)
following OECD Guideline 203.
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