Registration Dossier
Registration Dossier
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EC number: 203-696-6 | CAS number: 109-69-3
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
The key value of toxicity of 1-chlorobutane on microorganisms was determined as EC50(3h) of >1000 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
The key study for acute toxicity of 1-chlorobutane to activated sludge was conducted according to OECD-guideline 209 and to GLP under static conditions (Evonik-Degussa 2010). The effect of a concentration range of 10 to 1000 mg/L of test substance on the respiration rate of 9.6 mL synthetic sewage in a 120 mL inoculum suspension after 3 hours resulted in an EC50(3h) of >1000 mg/L.
Three supporting publications evaluated effects on Photobacterium, Nitrosomonas, methanogens, aerobic heterotrophs, Vibrio and Shk1 with resulting I/EC50 values ranging from 110 to 486 mg/L.
(Blum et al. 1991: Photobacterium IC50(5min)=480 mg/L; Nitrosomonas IC50(24h)=120 mg/L; methanogens IC50(96h)=110 mg/L; Aerobic heterotrophs (Microtox) IC50(49h)=230 mg/L; Ren et al. 2003, Shk1 EC50=278 mg/L; Ren & Frymier 2002, Shk1 calculated from log(1/EC50) in mM: EC50calc.(5 min) = 279.6 mg/L; Vibrio fisheri (Microtox assay) EC50calc.(5 min) = 485.8 mg/L)
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