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Toxicological information

Acute Toxicity: other routes

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Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1962
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: very old report only minimal information given
Cross-reference
Reason / purpose for cross-reference:
reference to same study

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Unnamed
Year:
1962
Report date:
1962
Reference Type:
other:
Title:
Unnamed
Year:
1979
Report date:
1979

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Carbodiimide was administered in an oily solution to male rats via intraperitoneal injection. The animals were observed for at least 5 days and clinical signs and mortality monitored.
GLP compliance:
no
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Bis(2,6-diisopropylphenyl)carbodiimide
EC Number:
218-487-5
EC Name:
Bis(2,6-diisopropylphenyl)carbodiimide
Cas Number:
2162-74-5
Molecular formula:
C25H34N2
IUPAC Name:
N,N'-bis(2,6-diisopropylphenyl)carbodiimide
Details on test material:
- Name of test material (as cited in study report): Carbodiimid 4175
- Physical state: solid (bright yellow to brown and practically odour-less crystalline substance)

Test animals

Species:
rat
Strain:
other: Albino, unspecified strain
Sex:
male
Details on test animals or test system and environmental conditions:
no further details available

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
other: oil, unspecified
Details on exposure:
no data
Doses:
0.01 g/kg (0.2% in oil)
0.025 g/kg (0.5% in oil)
0,05 g/kg (0.5% in oil)
0.10 g/kg (1% in oil)
0.25 g/kg (5% in oil)
0.5 g/kg (10% in oil)
No. of animals per sex per dose:
5 (male rats only)
Control animals:
not specified
Details on study design:
no further details

Results and discussion

Effect levelsopen allclose all
Sex:
male
Dose descriptor:
LC0
Effect level:
>= 0.01 mg/kg bw
Based on:
test mat.
Sex:
male
Dose descriptor:
LC50
Effect level:
ca. 0.2 mg/kg bw
Based on:
test mat.
Sex:
male
Dose descriptor:
LC100
Effect level:
0.5 mg/kg bw
Based on:
test mat.
Mortality:
0.01 g/kg: no mortality
0.025 g/kg: no mortality
0.05 g/kg: no mortality
0.1 g/kg: no mortality
0.25 g/kg: 4 dead rats
0.50 g/kg: 5 dead rats
Clinical signs:
0.01 g/kg: no clinical signs
0.025 g/kg: 5 animals with symptoms
0.05 g/kg: 5 animals with symptoms
0.10 g/kg: 5 animals with symptoms
0.25 g/kg: 5 animals with symptoms
0.50 g/kg: 5 animals with symptoms

Symptoms were similar as the symptoms which occurred after oral exposure (reduced general condition, spastic posture, expanded abdomen (start 1 hour after application, reversible within 3 days)).
Body weight:
no data
Gross pathology:
no data
Other findings:
no further details available

Applicant's summary and conclusion

Conclusions:
The study was considered to be of low reliability (reliability Klimisch 3), because of the limited information available. The test material did induce mortality and treatment-related clinical signs in the dose group of 500 mg/kg bw (100 % mortality) and in dose group 250 mg/kg bw (80 % mortality). In the dose groups 10, 25, 50 and 100 mg/kg no mortality was evident.
Executive summary:

The acute intraperitoneal toxicity of bis(2,6-diisopropylphenyl)-carbodiimide was investigated in rats (Kimmerle, 1962). The test item was administered in oil in concentrations of 10, 25, 50, 100, 250 and 500 mg/kg bw via intraperitoneal injection to male albino rats (groups of 5). Mortality occurred with all animals in the group dosed with 500 mg/kg bw. The symptoms were poor general condition, hunched posture and expanded abdomen. 4 animals died in the group dosed with 250 mg/kg bw. No mortality was found in the dose groups of 10, 25, 50 and 100 mg/kg bw. An LD50 of ca. 200 mg/kg bw was identified.