Bis(2,6-diisopropylphenyl)carbodiimide

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

other information

Study period:

1962

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: very old report, methodic deficiencies, only limited details available

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Unnamed

Year:

1962

Report date:

1962

Reference 2

Reference Type:

other:

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The experiments were conducted in a test room (2 m³), in which 1 rabbit, 2 guinea pigs, 5 rats and 10 mice were housed. The test item was solved in alcohol and sprayed once at the beginning of the experiment. This resulted in a considerable to heavy mist production. The test item was administered as vapour (whole body exposure) in concentrations of 1, 2.5 and 5 mg/L air for 1 hour. Observations were made for at least 28 days.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Bis(2,6-diisopropylphenyl)carbodiimide

EC Number:

218-487-5

EC Name:

Bis(2,6-diisopropylphenyl)carbodiimide

Cas Number:

2162-74-5

Molecular formula:

C25H34N2

IUPAC Name:

N,N'-bis[2,6-bis(propan-2-yl)phenyl]methanediimine

Details on test material:

- Name of test material (as cited in study report): Carbodiimid 4175
- Physical state: solid (bright yellow to brown and practically odour-less crystalline substance)

Test animals

Species:

other: rabbits, guinea pigs, rats and mice

Strain:

not specified

Sex:

not specified

Details on test animals or test system and environmental conditions:

The experiments were conducted in a test room (2 m³), in which 1 rabbit, 2 guinea pigs, 5 rats and 10 mice were housed. Each species was housed individually.

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

air

Details on inhalation exposure:

The test item was solved in alcohol and sprayed once at the beginning of the experiment. This resulted in a considerable to heavy mist production.

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

1 h

Concentrations:

1.0 mg/L air
2.5 mg/L air
5.0 mg/L air

No. of animals per sex per dose:

1 rabbit, 2 guineas pigs, 5 rats, 10 mice

Control animals:

not specified

Details on study design:

no further details

Results and discussion

Effect levels

Effect levels 1

Sex:

not specified

Dose descriptor:

LCLo

Effect level:

2.5 mg/L air (nominal)

Exp. duration:

1 h

Remarks on result:

other: for rats

Effect levels 2

Sex:

not specified

Dose descriptor:

LC50

Effect level:

>= 2.5 mg/L air (nominal)

Exp. duration:

1 h

Remarks on result:

other: for guinea pigs

Effect levels 3

Sex:

not specified

Dose descriptor:

LC100

Effect level:

>= 2.5 mg/L air (nominal)

Exp. duration:

1 h

Remarks on result:

other: for mice

Effect levels 4

Sex:

not specified

Dose descriptor:

LC50

Effect level:

ca. 1 mg/L air (nominal)

Exp. duration:

1 h

Remarks on result:

other: for mice

Mortality:

1 mg/L air: after exposure 7 mice died within a period of up to eight days.
2.5 mg/L air: after exposure 1 guinea pig, 1 rat and 10 mice died within a period of 14 days.
5.0 mg/L air: after exposure 1 guinea pig, 2 rats and 10 mice died within a period of up to eight days.

Clinical signs:

other: 1 mg/L air: no clinical signs during exposure, in the following 8 days reduced general condition was evident, recovery in the following 8 days. 2.5 mg/L air: reduced general condition already during exposure evident. 5 mg/L air: considerably reduced gene

Body weight:

no data

Gross pathology:

no data

Other findings:

no data

Applicant's summary and conclusion

Interpretation of results:

Toxicity Category II

Remarks:

Migrated information Criteria used for interpretation of results: other: EU-GHS

Conclusions:

The study was considered to be of low reliability (reliability Klimisch 3), because of the limited information available. The test material did induce mortality and treatment-related clinical signs in the dose group of 5 mg/L air (100 % mortality in mice, 40 % mortality in rats and 50 % mortality in guinea pigs) and in dose group 2.5 mg/L air (100 % mortality in mice, 20 % mortality in rats and 50 % mortality in guinea pigs). In the dose groups 1 mg/ L air mortality was also evident (70 % mortality in mice).

Executive summary:

The acute inhalatory toxicity of bis(2,6-diisopropylphenyl)-carbodiimide was investigated in 1 rabbit, 2 guinea pigs, 5 rats and 10 mice (Kimmerle, 1962). The test item was administered as vapour (whole body exposure) in concentrations of 1, 2.5 and 5 mg/L air for 1 hour. Observations were made for at least 28 days. The test material did induce mortality and treatment-related clinical signs in the dose group of 5 mg/L air (100 % mortality in mice, 40 % mortality in rats and 50 % mortality in guinea pigs) and in dose group 2.5 mg/L air (100 % mortality in mice, 20 % mortality in rats and 50 % mortality in guinea pigs). In the dose groups 1 mg/ L air mortality was also evident (70 % mortality in mice). The symptoms were poor general condition. So an LD50 value of ca. 1 mg/L air was derived for mice and an LD50 for guinea pigs of >= 2.5 mg/L air.

However, as the substance was first of all solved in alcohol before spraying, and secondly no analytics were performed, the results of this study are not reliable, and the methodical deficiencies do not allow the use of it. Therefore the results of this study are not considered for classification and labelling.