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Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
other information
Study period:
1962
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: very old report, methodic deficiencies, only limited details available
Cross-reference
Reason / purpose for cross-reference:
reference to same study

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Unnamed
Year:
1962
Report date:
1962
Reference Type:
other:
Title:
Unnamed
Year:
1979
Report date:
1979

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The experiments were conducted in a test room (2 m³), in which 1 rabbit, 2 guinea pigs, 5 rats and 10 mice were housed. The test item was solved in alcohol and sprayed once at the beginning of the experiment. This resulted in a considerable to heavy mist production. The test item was administered as vapour (whole body exposure) in concentrations of 1, 2.5 and 5 mg/L air for 1 hour. Observations were made for at least 28 days.
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Bis(2,6-diisopropylphenyl)carbodiimide
EC Number:
218-487-5
EC Name:
Bis(2,6-diisopropylphenyl)carbodiimide
Cas Number:
2162-74-5
Molecular formula:
C25H34N2
IUPAC Name:
N,N'-bis(2,6-diisopropylphenyl)carbodiimide
Details on test material:
- Name of test material (as cited in study report): Carbodiimid 4175
- Physical state: solid (bright yellow to brown and practically odour-less crystalline substance)

Test animals

Species:
other: rabbits, guinea pigs, rats and mice
Strain:
not specified
Sex:
not specified
Details on test animals or test system and environmental conditions:
The experiments were conducted in a test room (2 m³), in which 1 rabbit, 2 guinea pigs, 5 rats and 10 mice were housed. Each species was housed individually.

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
air
Details on inhalation exposure:
The test item was solved in alcohol and sprayed once at the beginning of the experiment. This resulted in a considerable to heavy mist production.
Analytical verification of test atmosphere concentrations:
no
Duration of exposure:
1 h
Concentrations:
1.0 mg/L air
2.5 mg/L air
5.0 mg/L air
No. of animals per sex per dose:
1 rabbit, 2 guineas pigs, 5 rats, 10 mice
Control animals:
not specified
Details on study design:
no further details

Results and discussion

Effect levelsopen allclose all
Sex:
not specified
Dose descriptor:
LCLo
Effect level:
2.5 mg/L air (nominal)
Exp. duration:
1 h
Remarks on result:
other: for rats
Sex:
not specified
Dose descriptor:
LC50
Effect level:
>= 2.5 mg/L air (nominal)
Exp. duration:
1 h
Remarks on result:
other: for guinea pigs
Sex:
not specified
Dose descriptor:
LC100
Effect level:
>= 2.5 mg/L air (nominal)
Exp. duration:
1 h
Remarks on result:
other: for mice
Sex:
not specified
Dose descriptor:
LC50
Effect level:
ca. 1 mg/L air (nominal)
Exp. duration:
1 h
Remarks on result:
other: for mice
Mortality:
1 mg/L air: after exposure 7 mice died within a period of up to eight days.
2.5 mg/L air: after exposure 1 guinea pig, 1 rat and 10 mice died within a period of 14 days.
5.0 mg/L air: after exposure 1 guinea pig, 2 rats and 10 mice died within a period of up to eight days.
Clinical signs:
other: 1 mg/L air: no clinical signs during exposure, in the following 8 days reduced general condition was evident, recovery in the following 8 days. 2.5 mg/L air: reduced general condition already during exposure evident. 5 mg/L air: considerably reduced gene
Body weight:
no data
Gross pathology:
no data
Other findings:
no data

Applicant's summary and conclusion

Interpretation of results:
Toxicity Category II
Remarks:
Migrated information Criteria used for interpretation of results: other: EU-GHS
Conclusions:
The study was considered to be of low reliability (reliability Klimisch 3), because of the limited information available. The test material did induce mortality and treatment-related clinical signs in the dose group of 5 mg/L air (100 % mortality in mice, 40 % mortality in rats and 50 % mortality in guinea pigs) and in dose group 2.5 mg/L air (100 % mortality in mice, 20 % mortality in rats and 50 % mortality in guinea pigs). In the dose groups 1 mg/ L air mortality was also evident (70 % mortality in mice).
Executive summary:

The acute inhalatory toxicity of bis(2,6-diisopropylphenyl)-carbodiimide was investigated in 1 rabbit, 2 guinea pigs, 5 rats and 10 mice (Kimmerle, 1962). The test item was administered as vapour (whole body exposure) in concentrations of 1, 2.5 and 5 mg/L air for 1 hour. Observations were made for at least 28 days. The test material did induce mortality and treatment-related clinical signs in the dose group of 5 mg/L air (100 % mortality in mice, 40 % mortality in rats and 50 % mortality in guinea pigs) and in dose group 2.5 mg/L air (100 % mortality in mice, 20 % mortality in rats and 50 % mortality in guinea pigs). In the dose groups 1 mg/ L air mortality was also evident (70 % mortality in mice). The symptoms were poor general condition. So an LD50 value of ca. 1 mg/L air was derived for mice and an LD50 for guinea pigs of >= 2.5 mg/L air.

However, as the substance was first of all solved in alcohol before spraying, and secondly no analytics were performed, the results of this study are not reliable, and the methodical deficiencies do not allow the use of it. Therefore the results of this study are not considered for classification and labelling.