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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well documented study report which meets basic scientific principles

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1981
Report date:
1981

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
Standardised test to assess the acute toxicity of the test substance after i.p. application in mice (BASF-Test).
GLP compliance:
no
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Anisaldehyde
EC Number:
204-602-6
EC Name:
Anisaldehyde
Cas Number:
123-11-5
Molecular formula:
C8 H8 O2
IUPAC Name:
4-methoxybenzaldehyde
Details on test material:
- Name of test material (as cited in study report): Anisaldehyd
- Physical state: liquid
- Analytical purity: > 99 % (data from order sheet)

Test animals

Species:
mouse
Strain:
NMRI
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: WIGA
- Mean weight per test group at study initiation: 22-28 g (males); 22-24 g (females)
- Fasting period before study: 15-20 h
- Diet: Herilan MRH-Haltung, H. Eggersmann KG ad libitum

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
CMC (carboxymethyl cellulose)
Remarks:
aqueous 0.5% CMC solution
Details on exposure:
VEHICLE
- Concentration in vehicle: 2% - 20%

MAXIMUM DOSE VOLUME APPLIED: 10 mL/kg
Doses:
200, 700, 2000 mg/kg bw
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Weighing was performed at the beginning of the study and on days 3-4, 7 and 13. Observation of clinical signs was several times on the day of administration and once daily afterwards with the exception of weekends and on holidays
- Necropsy of survivors performed: yes

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 700 - < 2 000 mg/kg bw
Based on:
test mat.
Mortality:
2000 mg/kg bw: 10/10
700 mg/kg bw: 0/10
200 mg/kg bw: 0/10
Clinical signs:
2000 mg/kg: dyspnoea, apathy, abnormal position, staggering, atonia, loss of cornea reflex, twitching, narcotic like state, poor general condition

700 mg/kg: dyspnoea, apathy, abnormal position, staggering, twitching, tremor, poor general condition

200 mg/kg: no adverse effects
Body weight:
The body weight gain was within the normal range.
Gross pathology:
Animals that died or were sacrificed after post observation period: no intra-abdominal substance precipitations or adhesions.

Applicant's summary and conclusion