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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable study which meets basic scientific principles; not according to current standart protocols

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1981
Report Date:
1981

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Based on Smyth H.F. et al.: Am. Ind. Hyg. Ass. J. 23, 95-107, (1962)
GLP compliance:
no
Test type:
other: Inhalation hazard test (IHT)
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Anisaldehyd
- Physical state: liquid
- Analytical purity: > 99 % (data from order sheet)

Test animals

Species:
rat
Strain:
not specified
Sex:
male/female

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
other: unchanged (no vehicle)
Details on inhalation exposure:
Rats were exposed for 7 h to a vapour saturated atmosphere.
Vapour was generated by bubbling 200 L/h dry air (no CO2) through the liquid test substance in a column (volume ca. 50 mL) of about 5 cm above a fritted glass disc in a glass cylinder. The glass cylinder was heated in a water bath. The air pressure was 770-772 mm Hg. Temperature in the exposure chamber was 20°C.
The nominal concentration of the test atmosphere was determined by reweighing of the substance. No further analytical measurements have been performed for determination of the test substance concentration.
Analytical verification of test atmosphere concentrations:
no
Duration of exposure:
7 h
Concentrations:
0.32 mg/L (57.53 ppm)
No. of animals per sex per dose:
6
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Weighing was done only on the day of exposure. Observations were done several times at the day of exposure and daily with the exception of weekends and holidays afterwards.
- Necropsy of survivors performed: yes
Statistics:
not performed

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
other: IHT (inhalation hazard test)
Effect level:
0.32 mg/L air (nominal)
Based on:
test mat.
Exp. duration:
7 h
Remarks on result:
other: 0/12 animals died after exposure to a saturated vapour atmosphere for 7 h
Mortality:
0/12 animals died
Clinical signs:
other: During exposure, snout wiping and attempts to escape were observed.
Body weight:
Not reported
Gross pathology:
No abnormalities

Applicant's summary and conclusion