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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions. The study is sufficient for the evaluation of ethylchloroformate

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1970
Report date:
1970

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
Deviations:
yes
Remarks:
: low volume of test article; does, however, not invalidate the result.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Ethyl chloroformate
EC Number:
208-778-5
EC Name:
Ethyl chloroformate
Cas Number:
541-41-3
Molecular formula:
C3H5ClO2
IUPAC Name:
ethyl chlorocarbonate
Details on test material:
- Name of test material (as cited in study report): Aethylchlorkohlensäureester (ethy carbonochloric acid ester)
- Substance type:
- Physical state: liquid
- Analytical purity: ca. 95 %
- internalTest substance No. XIX/351.

Test animals / tissue source

Species:
rabbit
Strain:
Vienna White
Details on test animals or tissues and environmental conditions:
TEST ANIMALS
- Source: Gaukler, Offenbach, Germany
- Weight at study initiation: 2.0 kg (f), 2.0 kg (m), 3.0 kg (m)
- Housing: single
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum

ENVIRONMENTAL CONDITIONS
no data

Test system

Vehicle:
unchanged (no vehicle)
Controls:
not required
Amount / concentration applied:
0.05 ml
Duration of treatment / exposure:
application ito the conjunctival sack, no washing afterwards
Observation period (in vivo):
8 days
Number of animals or in vitro replicates:
4
Details on study design:
REMOVAL OF TEST SUBSTANCE
- Washing (if done): no washing done

SCORING SYSTEM:
Comparable to the draize scoring system

TOOL USED TO ASSESS SCORE: luorescein

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
other: 24 - 72 h
Score:
2
Max. score:
3
Reversibility:
not reversible
Irritation parameter:
iris score
Basis:
mean
Time point:
other: 24 - 72 h
Score:
1.4
Max. score:
2
Reversibility:
not reversible
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: 24 - 72 h
Score:
1.7
Max. score:
2
Reversibility:
not reversible
Irritation parameter:
chemosis score
Basis:
mean
Time point:
other: 24 - 72 h
Score:
2.8
Max. score:
4
Reversibility:
not reversible
Irritant / corrosive response data:
Severe eye irritation and corneal opacity was seen at 1 h after application and persisted until the end of the observation period on day 8 after treatment.

Any other information on results incl. tables

Descriptive scores of the raw data have been converted to Draize numerical scores.

Animal

Reading

Opacity

Iritis

Redness

Chemosis

Comment

1

1 h

3

0

2

2

 

2

1 h

1

0

2

3

 

3

1 h

3

0

1

2

 

4

1h

3

0

2

4

 

1

24 h

3

0

2

2

hemorrhage

2

24 h

2

0

2

3

 

3

24 h

3

0

1

3

 

4

24 h

3

2

2

3

hemorrhage

1

48 h

2

2

2

2

hemorrhage

2

48 h

-

-

-

-

no data

3

48 h

-

-

-

-

no data

4

48 h

2

2

2

3

hemorrhage

1

72 h

2

2

2

3

hemorrhage

2

72 h

2

2

2

3

 

3

72 h

3

2

1

3

 

4

72 h

2

2

2

3

hemorrhage

1

8 d

3

2

2

1

staphyloma/suppuration

2

8 d

3

2

2

1

staphyloma/suppuration

3

8 d

3

2

2

1

staphyloma/suppuration

4

8 d

3

2

2

1

staphyloma/suppuration

mean

24 - 72 h

2,4

1.4

1.7

2.80

 

 

Applicant's summary and conclusion

Interpretation of results:
corrosive
Remarks:
Migrated information
Conclusions:
The results indicate the risk of serious damage to eyes.