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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Due to lower dokumentation requirements in times before GLP the study is reliable with restrictions.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1970
Report date:
1970

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Deviations:
yes
GLP compliance:
no
Test type:
standard acute method

Test material

Constituent 1
Chemical structure
Reference substance name:
Ethyl chloroformate
EC Number:
208-778-5
EC Name:
Ethyl chloroformate
Cas Number:
541-41-3
Molecular formula:
C3H5ClO2
IUPAC Name:
ethyl chlorocarbonate
Details on test material:
- Name of test material (as cited in study report): Aethylchlorkohlensäureester (ethy carbonochloric acid ester)
- Substance type:
- Physical state: liquid
- Analytical purity: ca. 95 %
- internalTest substance No. XIX/351.

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Gassner
- Weight at study initiation: 216,9 +/- 27.7 (males); 166,8 +/-15,3 (females
- Fasting period before study: no data (probably: yes)
- Housing: no data (probably: grousp of 5)
- Diet (e.g. ad libitum): no data (probably: ad libitum)
- Water (e.g. ad libitum): no data (probably: ad libitum)
- Acclimation period:no data


ENVIRONMENTAL CONDITIONS
not reported

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
water
Details on oral exposure:
VEHICLE
- Vehicle aqueous solution with 0.1 -1 % traganth gum
- Concentration in vehicle: 1 %

MAXIMUM DOSE VOLUME APPLIED: ca. 10 ml
Doses:
80, 100, 125, 160, 200,250, 320, 400 µl/kg

No. of animals per sex per dose:
10
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Observations were several times the day of application and at least daily thereafter.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs
Statistics:
none

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
ca. 205 mg/kg bw
Remarks on result:
other: desity 1,14 g/cm3
Mortality:
400 µl/kg: 10/10 male and 10/10 female (within the first 24 h)
320 µl/kg: 10/10 male and 10/10 female (with the exeption of one male all within the first 24 h)
250 µl/kg: 5/10 male and 10/10 female (all death occurred within the first 48 h)
200 µl/kg: 5/10 male and 8/10 female
160 µl/kg: 2/10 male and 1/10 female
125 µl/kg: 0/10 male and 6/10 female
100 µl/kg: 0/10 male and 6/10 female
80 µl/kg: 0/10 male and 2/10 female
Clinical signs:
Dyspnea; apathy; staggering; abdominal and lateral position; sometimes twitching
Body weight:
not determined at the end of the study
Gross pathology:
Animals founfd dead: Dark-red discoloration of the gastric mucosa; reddish intestinal contents; serous membranes rich in liquids. 2 x lung edema
Sacrificed animals: nothing abnormal

Any other information on results incl. tables

LD 50 was estimated to be 180 µL/kg. this is equivalent to 205 mg/kg bw based on a density of 1.14 g/cm³ at 20°C

Signs of toxicity: dyspnea; apathy; staggering; abdominal and lateral position; sometimes twitching.

Necropsy findings: dark-red discoloration of the gastric mucosa; reddish intestinal contents; serous membranes rich in liquids.

Applicant's summary and conclusion