Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP-guideline study with analytical monitoring

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report Date:
2003

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Tridecanol A
- Physical state: liquid/colourless-clear
- Analytical purity: 99.95%

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: All test concentrations were prepared separately. The test substance was injected by a microtiter-syringe in the test water. All concentrations were treated in an ultrasonic bath for about 3 min. and subsequently stirred for about 20 hours at 20 ± 2 °C.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Source: Institut National de Recherche Chimique Appliquee, France), cultured under standard laboratory conditions
- Age at study initiation: 2 - 24 hours

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.2 - 3.2 mmol/L
Test temperature:
20 ± 2 °C
pH:
7.5 - 8.5
Dissolved oxygen:
- M4 medium is aerated for about 24 hours until saturation with oxygen is reached
Nominal and measured concentrations:
Nominal test concentrations: control, 0.156, 0.313, 0.625, 1.25, 2.5, 5.0 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, total volume, fill volume: glass test tubes with flat bottom, 20 ml, 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 5
- Biomass loading rate: 0.5 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Alkalinity: 0.8 - 1.0 mmol/L (up to pH 4.3)
- Ca/mg ratio: about 4:1
- Conductivity: 550 - 650 µS/cm²
- Culture medium different from test medium: No, synthetic culture medium M4 based on ultra pure, deionised water
- Intervals of water quality measurement:
- Temperature: continuously during the whole test period in a separate vessel close
- After 0 h in an additional replicate without daphnids and after 48 h in replicate 1 of each tested concentration the test vessels

OTHER TEST CONDITIONS
- Photoperiod: 16 h light, 8 h darkness
- Light intensity: artificial light with an intensity of about 1 - 2 µE/(m*m*s)

EFFECT PARAMETERS MEASURED: mobility recorded after 0, 24 and 48 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
0.105 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.275 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL: 0.174 - 0,342 mg/L
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
1.13 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

Results on the basis of nominal concentrations:
EC0 (48h) = 0.156 mg/L
EC50 (48h) = 0.263 mg/L (CL 95%: 0.0324 - 0.585 mg/L)
EC100 (48h) = 1.25 mg/L

Applicant's summary and conclusion