2,6-xylidine

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Data from reliable source, usually GLP-guideline studies, detailed documentation available only in japanese.

Data source

Reference

Reference 1

Reference Type:

other: J-CHECK

Title:

Unnamed

Year:

2019

Report date:

2019

Reference 2

Reference Type:

other: Japanese database

Title:

Unnamed

Year:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,6-xylidine

EC Number:

201-758-7

EC Name:

2,6-xylidine

Cas Number:

87-62-7

Molecular formula:

C8H11N

IUPAC Name:

2,6-dimethylaniline

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

sampling intervals: fresh and aged test solutions on days of renewal (3 times per week: Monday, Wednesday, Friday)

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test conditions

Test temperature:

20±1 °C

Nominal and measured concentrations:

nominal values: control, 1.0, 2.2, 4.6, 10, 22 mg/L (geometric ratio: 2.2)

Details on test conditions:

TEST SYSTEM
- Material, size, headspace, fill volume: glass, fill volume 80 mL
- Renewal rate of test solution (frequency/flow rate): 3 times per week
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: room light, 16 hours light - 8 hours dark

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

2.23 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

act. ingr.

Basis for effect:

reproduction

Remarks on result:

other: Effect concentration was calculated based on time-weighted average values of 21 days which were determined based on logarithmic average values calculated from measured values of renewal and old test solution.

Effect concentrations 2

Key result

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

6.54 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

act. ingr.

Basis for effect:

reproduction

Remarks on result:

other: 95 % CL: 6.38 - 6.70 mg/L

Remarks:

Effect concentration was calculated based on time-weighted average values of 21 days which were determined based on logarithmic average values calculated from measured values of renewal and old test solution.

Effect concentrations 3

Duration:

21 d

Dose descriptor:

LC50

Effect conc.:

12.1 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

act. ingr.

Basis for effect:

mortality

Remarks on result:

other: 95% CL: 9.43 – 16.7 mg/L

Remarks:

Effect concentration was calculated based on time-weighted average values of 21 days which were determined based on logarithmic average values calculated from measured values of renewal and old test solution.

Effect concentrations 4

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

4.59 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

act. ingr.

Basis for effect:

reproduction

Remarks on result:

other: Effect concentration was calculated based on time-weighted average values of 21 days which were determined based on logarithmic average values calculated from measured values of renewal and old test solution.