Benzenesulfonic acid, 4-C10-13-sec-alkyl derivs., compds. with 2-propanamine

Toxicity to terrestrial plants

Administrative data

Endpoint:

toxicity to terrestrial plants: long-term

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

April 23, 1987-June 8, 1987

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Data source

Reference

Reference 1

Reference Type:

other company data

Title:

Unnamed

Year:

1987

Report date:

1987

Reference 2

Reference Type:

other company data

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)

Deviations:

yes

Remarks:

% particles less than 20 µg not given; 4.1% instead of maximum 3% organic carbon in the test soil; sand was sieved with 1 mm mesh instead of 0.5 mm mesh; weight and variability of seeds not reported.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts

EC Number:

270-115-0

EC Name:

Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts

Cas Number:

68411-30-3

Molecular formula:

Not applicable for UVCB

IUPAC Name:

sodium 4-undecylbenzenesulfonate

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

not specified

Details on preparation and application of test substrate:

The test was conducted in an artificial soil consisting of commercially available potting compost (WC-B) and washed, sieved (1 mm mesh), dried (temperature and time not reported) silver sand (1:9 potting soil:washed sand).

Test organisms

1

Species:

Sorghum bicolor

Plant group:

Monocotyledonae (monocots)

Details on test organisms:

- Common name: sorghum
- Variety: Dekalb 64
- Source of seed: Dekalk, USA
- Historical germination of seed (germination of seed lot tested): viability of at least 80%

2

Species:

Helianthus annuus

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: sunflower
- Variety: Rodeo
- Source of seed: Codasal, France
- Historical germination of seed (germination of seed lot tested): viability of at least 80%

3

Species:

Phaseolus aureus

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: mung bean
- Source of seed: Clause Seeds, UK
- Historical germination of seed (germination of seed lot tested): viability of at least 80%

Study design

Test type:

seedling emergence toxicity test

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

21 d

Test conditions

Test temperature:

11-37 degrees C

pH:

6.8-7.9

Moisture:

11.9%

Details on test conditions:

TEST SYSTEM
- Testing facility: Jealott's Hill Greenhouse Number 130/2
- Test container (type, material, size): 10-cm diameter, non-porous plastic plant pots, capacity of approx. 600 g
- Amount of soil: ~600 g
- No. of seeds per container: 8
- No. of replicates per treatment group: 4
- No. of replicates per control: 4

GROWTH CONDITIONS
- Photoperiod: at least 14 hrs
- Light source: if natural sunlight was poor, mercury vapor lamps were used
- Light intensity and quality: 5,000 lux
- Watering regime and schedules: capillary matting sub-irrigation system, twice daily

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : seedling emergence

Nominal and measured concentrations:

0, 1, 10, 100, 1000 mg/kg active ingredient in dry soil

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Species:

other: all 3 species

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth

Effect concentrations 2

Species:

Sorghum bicolor

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

167 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth

Effect concentrations 3

Species:

Helianthus annuus

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

289 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth

Effect concentrations 4

Species:

Phaseolus aureus

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

316 mg/kg soil dw

Nominal / measured:

meas. (initial)

Conc. based on:

act. ingr.

Basis for effect:

growth

Effect concentrations 5

Species:

Sorghum bicolor

Duration:

21 d

Dose descriptor:

EC10

Effect conc.:

68 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth

Effect concentrations 6

Species:

Helianthus annuus

Duration:

21 d

Dose descriptor:

EC10

Effect conc.:

116 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth

Effect concentrations 7

Species:

Phaseolus aureus

Duration:

21 d

Dose descriptor:

EC10

Effect conc.:

126 mg/kg soil dw

Nominal / measured:

meas. (initial)

Conc. based on:

act. ingr.

Basis for effect:

growth

Details on results:

There was 91% emergence of sunflowers at all concentrations. Mung bean emergence was at least 84% at concentrations of 100 mg/kg AI or below. At 1000 mg/kg AI, emergence was only 75%. Seed emergence was 78-84% in sorghum for controls and all concentrations but the highest. At 1,000 mg/kg AI, emergence was only 69%. At the 1000 mg/kg AI concentration, sunflowers and mung beans barely emerged, and showed signs of dying. Sorghum shoots at this concentration were healthy but stunted. The low emergence in mung beans may be due to a cold spell early in the test.

Any other information on results incl. tables

Number (Percent) of Plants Emerged at Day 7

Species	Control	1 mg/kg	10 mg/kg	100 mg/kg	1000 mg/kg
Sorghum	26 (81)	27 (84)	27 (84)	25 (78)	22 (69)
Sunflower	30 (94)	32 (100)	30 (94)	32 (100)	29 (91)
Mung bean	31 (97)	27 (84)	30 (94)	27 (84)	24 (75)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The NOEC for all three species is 100 mg/kg AI. The 21-day EC50 for sunflower was 289 mg/kg AI, for sorghum it was 167 mg/kg AI, and for mung bean it was 316 mg/kg AI.

Executive summary:

The toxicity of the test substance to three species of plant, sunflower, sorghum, and mung bean, was determined. Seeds of these three species were grown in soil containing concentrations of the test ingredient of 0, 1, 10, 100, 1000 mg/kg active ingredient in dry soil. The emergence and growth of seedlings was monitored for 21 days. There was a decrease in emergence of all three species at the highest concentration, with some shoots barely emerging, and stunted and dying. The NOEC for all three species is 100 mg/kg AI. The 21-day EC50 for sunflower was 289 mg/kg AI, for sorghum it was 167 mg/kg AI, and for mung bean it was 316 mg/kg AI.