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Diss Factsheets

Ecotoxicological information

Long-term toxicity to fish

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Administrative data

fish life cycle toxicity
Type of information:
experimental study
Adequacy of study:
weight of evidence
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Referenceopen allclose all

Reference Type:
Reference Type:
Predicted no-effect concentrations and risk characterization of four surfactants: Linear alkylbenzene sulfonate, alcohol ethoxylates, alcohol ethoxylated sulfates, and soap.
van de Plassche, E.J., de Bruijn, J.H.M., Stephenson, R.R., Marshall, S.J., Feijtel, T.C.J. and Belanger, S.E.
Bibliographic source:
Environmental Toxicology and Chemistry. 18:2653-2663.

Materials and methods

Principles of method if other than guideline:
During the first four months a serial-dilution unit was used to maintain the LAS at the desired concentration in System I and a proportional dilution unit was used in System II, both to provide continuous exposures to fathead minnows. During the latter part of the test, the flows from a new proportional dilution unit were divided to supply both exposure systems and a fry exposure system.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts
EC Number:
EC Name:
Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts
Cas Number:
Molecular formula:
Not applicable for UVCB
sodium 4-undecylbenzenesulfonate
Details on test material:
LAS, activity: 60.8%; equivalent MW = 348

Sampling and analysis

Analytical monitoring:

Test solutions

not specified

Test organisms

Test organisms (species):
Pimephales promelas
Details on test organisms:
Fish were obtained from the ponds at the Newtown Fish Farm, Ohio Division of Wildlife. The small young-of-the-year fish were collected in late September and held in cold water. During the last week of December, 25 fish were placed randomly into each exposure chamber where they were maintained in a continuous flow of dilution water similar to that used during the test. The four largest and eight smallest fish from each aquarium were selected in order to reduce the number of fish and to try to get a more equal distribution of sexes. The exposure to LAS was started during the last week of February.

Study design

Test type:
other: other, open-system
Water media type:
Limit test:
Total exposure duration:
196 d

Test conditions

water hardness ranged from 194-214 mg/L CaCO3
pH ranged from 7.50-7.95
Dissolved oxygen:
Mean dissolved oxygen 5.84-6.42 mg/L
Nominal and measured concentrations:
Test concentrations were 0.34, 0.63, 1.2 and 2.7 mg/L.
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Key result
196 d
Dose descriptor:
Effect conc.:
0.63 mg/L
Nominal / measured:
meas. (geom. mean)
Basis for effect:
other: fry survival
196 d
Dose descriptor:
Effect conc.:
1.2 mg/L
Nominal / measured:
meas. (geom. mean)
Basis for effect:
other: fry survival
Details on results:
Hatchability and growth were not significantly affected. This study was on C12-LAS and when normalized to C11.6, the value is 0.90 mg/L (van de Plassche et al., 1999).

Applicant's summary and conclusion

Validity criteria fulfilled:
The 196 day NOEC level was 0.63 mg/L. The LOEC was 1.2 mg/L.
Executive summary:

Fathead minnows were exposed to LAS in continuous flow systems for a total of 196 days. Results indicate that lethality of LAS to newly hatched fry was the most critical factor found.