Didodecyl 3,3'-thiodipropionate

Toxicity to reproduction: other studies

Administrative data

Endpoint:

toxicity to reproduction: other studies

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable study report which meets basic scientific principles.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Dilauryl Thiodipropionate was tested for toxic and teratogenic effects to the developing chicken embryo.

GLP compliance:

not specified

Type of method:

in vivo

Test material

Test material information

Constituent 1

Details on test material:

- Name of test material (as cited in study report): Dilauryl Thiodipropionate
No further details.

Test animals

Species:

other: chicken egg

Strain:

not specified

Details on test animals or test system and environmental conditions:

not applicable

Administration / exposure

Route of administration:

other: via air cell or via the yolk

Vehicle:

other: sesame oil

Details on exposure:

The test substance was administered in sesame oil by the two routes at two stages of embryonic development: via the air cell at pre-incubation (0 hours) and at 96 hours of incubation, and via the yolk at 0 hours and at 96 hours.

Duration of treatment / exposure:

0 or 96 hours

Frequency of treatment:

single treatment

Duration of test:

until the chicks hatched

Doses / concentrations

Remarks:

Doses / Concentrations:
0.25, 0.5, 2.5, 5, 10 mg/egg (corresponding to 5, 10, 50, 100, 200 mg/kg)
Basis:
nominal conc.

No. of animals per sex per dose:

10 or more (not further specified)

Control animals:

yes, concurrent vehicle

Details on study design:

The test substance was tested for toxic and teratogenic effects to the developing chicken embryo under four sets of conditions. It was administered in sesame oil as the solvent by the two routes at two stages of embryonic development: via the air cell at pre-incubation (0 hours) and at 96 hours of incubation, and via the yolk at 0 hours and at 96 hours using techniques that have been described previously (McLaughlin, J., Jr., Marliac, J.-P., Verrett, M. Jacqueline, Mutchler,
Mary K., and Fitzhugh, 0. G., (1963) Toxicol. Appl. Pharmacol. 5, 760-770; Verrett, M. J., Marliac, J.-P., and McLaughlin, J., Jr., (1964) JADAC 47, 1003-1006.).
Groups of 10 or more eggs were treated under these four conditions at several dose levels until a total of ninety to one hundred eggs per level was reached for all levels allowing some to hatch. Groups of comparable size were treated with the solvent at corresponding volumes and untreated controls were also included in each experiment.
After treatment, all eggs were candled daily and non-viable embryos removed. Surviving embryos were allowed to hatch. All hatched chicks and non-viable embryos were examined carefully for abnormalities (internally and externally) as well as for toxic responses (such as oedema, haemorrhage, hypopigmentation of the down and other disorders such as feather abnormalities, significant groivth retardation, cachexia, ataxia or other nerve disorder; structural abnormality of the head, viscera, limbs, or body skeleton) and accidental deaths.
At hatchings, 3 chicks were removed at random from each level including control for skeletal clearing, weighing and fixing of bursa, spleen, liver and kidney. Tissues were processed, blocked in paraffin, sectioned, affixed to slides, and stained. Later these sections were examined for internal damage to the tissues.

Statistics:

Chi Square Test.

Results and discussion

Observed effects

Significantly higher mortality rates were observed when the test substance was administered via yolk at the two stages of embryonic development. Air cell treatment at 0 hours was more toxic than the air cell treatment at 96 hours of embryonic development. There were no teratogenic effects observed in all the four test conditions employed.