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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
05-1989
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non GLP no reference substance and no analysis. However the test is well documented and the guideline was valid at that time. The data provided and the study design is enough to consider K2 as reliability.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Algen-Wachstumshemmtest DIN 38412/9
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2-chloroethanol
EC Number:
203-459-7
EC Name:
2-chloroethanol
Cas Number:
107-07-3
Molecular formula:
C2H5ClO
IUPAC Name:
2-chloroethan-1-ol
Details on test material:
2-chloroethanol
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Substance is a colorless liquid and is very soluble in water, boilingpoint is 129 C and the pH <7, the substance is stable under ambient conditions

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
cultivated at pH 8.2+-0.3 in 250 ml lage Erlemeyer with 100ml medium

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
20
pH:
8.3-9.2
Nominal and measured concentrations:
nominal: 0.2, 0.4, 0.8, 1.6, 3.2, 6.4 mg/L
Details on test conditions:
start with 10000 cells per ml. the test solution was transfered to 10ml bekers with as much solution. Illumination was at 120uE/m2s directed from above
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
0.95 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
5.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC90
Effect conc.:
30.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Reported statistics and error estimates:
mono variable linear regression and graphical analysis

Any other information on results incl. tables

 Concentration mg/L  Biomass  growthrate
 0  1.6  1.16
 0.20  1.54  1.12
 0.40  1.43  1.12
 0.80  1.20  1.06
 1.60  0.94  0.97
 3.20  0.67  0.70
 6.40  0.63  0.57

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
Study is valid however concentration is based on nominal. no reference substance is used so the control cannot be done. Therefore the use of this study is limited when applying it in a riskassessment
Executive summary:

An algal essay was performed according to the DIN 38412/9 Guideline with 2 -chloroethanol. The study design was static with 72h exposure. No reference substance was used. pH varied between 8.3 -9.2 and this range is acceptable especially in an algal essay. the EC10 for growth rate is to be sued when applying the result in a riskassessment and was determined via statistical extrapolation to be 0.95 mg/L of nominal test substance concentration.