Registration Dossier

Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Referenceopen allclose all

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
(Q)SAR
Adequacy of study:
key study
Study period:
Not applicable, calculated value
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: This summary has a reliability of 2 because the results are estimated using a computer model that is appropriate for use with this hydrocarbon substance.
Justification for type of information:
A discussion and report on the read across strategy is given as an attachment in IUCLID Section 13.
Reason / purpose:
read-across: supporting information
Qualifier:
no guideline followed
Principles of method if other than guideline:
The aquatic toxicity was estimated by a QSAR, the Petrotox computer model. This model combines a partitioning model used to calculate the aqueous concentration of hydrocarbon components with the Target Lipid Model used to calculate acute and chronic toxicity of non-polar narcotic chemicals. Petrotox computes toxicity based on the summation of the aqueous-phase concentrations of hydrocarbon block(s) that represent a hydrocarbon substance and membrane-water partition coefficients (KMW) that describe the partitioning of the hydrocarbons between the water and organism.
GLP compliance:
no
Remarks:
The data were calculated by a computer model
Analytical monitoring:
not required
Vehicle:
no
Test organisms (species):
Daphnia magna
Test type:
other: QSAR modeled data
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d
Key result
Duration:
21 d
Dose descriptor:
NOELR
Effect conc.:
0.162 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Validity criteria fulfilled:
yes
Conclusions:
The estimated freshwater invertebrate 21-day NOELR (No Observed Effect Loading Rate) value is 0.162 mg/L based on reproduction.
Executive summary:

The aquatic toxicity was estimated using the Petrotox computer model, which combines a partitioning model used to calculate the aqueous concentration of hydrocarbon components as a function of substance loading with the Target Lipid Model used to calculate acute and chronic toxicity of non-polar narcotic chemicals. Petrotox computes toxicity based on the summation of the aqueous-phase concentrations of hydrocarbon block(s) that represent a hydrocarbon substance and membrane-water partitioning coefficients (KMW) that describe the partitioning of the hydrocarbons between the water and organism. The estimated freshwater invertebrate 21-day NOELR (No Observed Effect Loading Rate) value is 0.162 mg/l based on reproduction.

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
19-Mar-2003 to 9-Apr-2003
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
The study was given a reliability rating of 1 because it followed a standard test guideline, which describes a procedure to evaluate this endpoint, and the results were reviewed for reliability and assessed as valid. The study was also conducted under GLP.
Justification for type of information:
A discussion and report on the read across strategy is given as an attachment in IUCLID Section 13.
Reason / purpose:
read-across: supporting information
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
Principles of method if other than guideline:
Followed Part 2 of OECD 202 test guideline.
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
Samples were removed from the Control and test concentrations 1000, 2500, 5000, and 10000 mg/l at test initiation (0-hours) and at 48-hours of the preliminary test. Samples were also removed from the control and 2500 and 5000 mg/l concentrations on Day 0, 7, 14, and 20 of the main study. Samples were stored at -18deg C until analysis.
Vehicle:
no
Details on test solutions:
Test solutions were prepared as Water Accommodated Fractions (WAF). The test substance was added to daphnia media, and stirred for 23 hours with a magnetic stirrer until equilibrium. The mixing was done at a slow enough speed as to not cause dispersion or emulsification of the undissolved test substance. The solution was allowed to stand for approximately 1 hour, to allow phase separation, before being carefully siphoned out via a glass tube (added prior to the addition of the test substance). Renewal of the test WAF was conducted every 48 hours.
Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna - Clone 5 (Crustacea, Daphniidae)
Origin - German Environmental Ministry
Pre-treatment - the animals are cultivated in a climate controlled room
(light/dark = 14h / 10h; illumination: GroLux fluorescent tubes).
The animals were fed with algae (Desmodesmus subspicatus).
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d
Test temperature:
19.7 to 20.8 deg C
pH:
8.0 to 8.2
Dissolved oxygen:
8.3 to 8.8 mg/l
Salinity:
freshwater
Nominal and measured concentrations:
Nominal concentrations were: 0, 2500, 5000 mg/l.

Concentration analysis (TOC) of the control and 2500 and 5000 mg/l test concentrations gave results of <10 mg/l. Indicating very low solubility of the test substance.
Details on test conditions:
40 animals/conc. (10 each per 4 replicates)
400 ml of solution in 800 ml glass beakers.
14h light / 10h dark - GroLux fluorescent tubes.
Animals were fed daily with algae (Desmodesmus subspicatus)
Reference substance (positive control):
no
Duration:
21 d
Dose descriptor:
EL50
Effect conc.:
> 5 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
immobilisation
Remarks:
Parental generation
Duration:
21 d
Dose descriptor:
EL50
Effect conc.:
< 2 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
NOELR
Effect conc.:
< 2 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Key result
Duration:
21 d
Dose descriptor:
EL50
Effect conc.:
ca. 1 400 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: inhibition of reproduction
Key result
Duration:
21 d
Dose descriptor:
NOELR
Effect conc.:
ca. 17 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: inhibition of reproduction
Details on results:
Immobilisation of Parental Generation: (at end of test)
Control - 10%
2500 mg/l - 20%
5000 mg/l - 28%

Date of first appearance of young (F1):
Control - 8d
2500 mg/l - 10d
5000 mg/l - 12d

Number of living young produced (at end of test):
Control - 712
2500 mg/l - 265 (62.8% inhibition of reproduction vs. control)
5000 mg/l - 177 (75.1% inhibition of reproduction vs. control)

Number of dead young produced (at end of test):
Control - 10
2500 mg/l - 16
5000 mg/l - 27
Reported statistics and error estimates:
Graphic evaluation on probability paper gave approximate results of the inhibition of reproduction.
Validity criteria fulfilled:
yes
Conclusions:
The chronic toxicity of Hydrocarbons, C16-C20, n-alkanes, isoalkanes, cyclics, aromatics (2 -30%) as measured by reproductive success to the water flea (Daphnia magna) was evaluated in freshwater. Hydrocarbons, C16-C20, n-alkanes, isoalkanes, cyclics, aromatics (2 -30%) showed toxic effects as a Water Accommodated Fraction (WAF) with a 21 -day EL50 of approximately 1400 mg/l and an NOELR of 17 mg/l (based on inhibition of reproduction).
Executive summary:

The chronic toxicity of Hydrocarbons, C16-C20, n-alkanes, isoalkanes, cyclics, aromatics (2 -30%) as measured by reproductive success to the water flea (Daphnia magna) was evaluated in freshwater. Hydrocarbons, C16-C20, n-alkanes, isoalkanes, cyclics, aromatics (2 -30%) showed toxic effects as a Water Accommodated Fraction (WAF) with a 21 -day EL50 of approximately 1400 mg/l and an NOELR of 17 mg/l (based on inhibition of reproduction).

Description of key information

Hydrocarbons, C16-C20, n-alkanes, isoalkanes, cyclics, aromatics (2-30%) did not cause toxicity in a 21-day toxicity study with Daphnia magna following standard test guidelines at a nominal loading of 1400 mg/l, with a NOELR of 17 mg/l (based on inhibition of reproduction). These results indicate that hydrocarbons, C16-C20, n-alkanes, isoalkanes, cyclics, aromatics (2-30%) is not expected to be chronically toxic to freshwater invertebrates. These data are used as read-across data to hydrocarbons, C14 -C18, n-alkanes, isoalkanes, cyclics, aromatics (2 -30%).

The estimated freshwater invertebrate 21-day NOELR (No Observed Effect Loading Rate) value for Hydrocarbons, C16-C20, n-alkanes, isoalkanes, cyclics, aromatics (2-30%) determined using the Petrotox computer model (CONCAWE, 2010) is 0.162 mg/L in Daphnia magna, based on reproduction.

Key value for chemical safety assessment

Additional information

Hydrocarbons, C16-C20, n-alkanes, isoalkanes, cyclics, aromatics (2-30%)did not cause toxicity in a 21-day toxicity study with Daphnia magna following standard test guidelines at a nominal loading of 1400 mg/l, with a NOELR of 17 mg/l (based on inhibition of reproduction).These results indicate that hydrocarbons, C16-C20, n-alkanes, isoalkanes, cyclics, aromatics (2-30%) is not expected to be chronically toxic to freshwater invertebrates.These data are used as read-across data to hydrocarbons, C14 -C18, n-alkanes, isoalkanes, cyclics, aromatics (2 -30%).

The estimated freshwater invertebrate 21-day NOELR (No Observed Effect Loading Rate) value for Hydrocarbons, C16-C20, n-alkanes, isoalkanes, cyclics, aromatics (2-30%) determined using the Petrotox computer model (CONCAWE, 2010) is 0.162 mg/L in Daphnia magna, based on reproduction.