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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
19-Mar-2003 to 9-Apr-2003
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
The study was given a reliability rating of 1 because it followed a standard test guideline, which describes a procedure to evaluate this endpoint, and the results were reviewed for reliability and assessed as valid. The study was also conducted under GLP.
Justification for type of information:
A discussion and report on the read across strategy is given as an attachment in IUCLID Section 13.
Reason / purpose for cross-reference:
read-across: supporting information
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
Principles of method if other than guideline:
Followed Part 2 of OECD 202 test guideline.
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
Samples were removed from the Control and test concentrations 1000, 2500, 5000, and 10000 mg/l at test initiation (0-hours) and at 48-hours of the preliminary test. Samples were also removed from the control and 2500 and 5000 mg/l concentrations on Day 0, 7, 14, and 20 of the main study. Samples were stored at -18deg C until analysis.
Vehicle:
no
Details on test solutions:
Test solutions were prepared as Water Accommodated Fractions (WAF). The test substance was added to daphnia media, and stirred for 23 hours with a magnetic stirrer until equilibrium. The mixing was done at a slow enough speed as to not cause dispersion or emulsification of the undissolved test substance. The solution was allowed to stand for approximately 1 hour, to allow phase separation, before being carefully siphoned out via a glass tube (added prior to the addition of the test substance). Renewal of the test WAF was conducted every 48 hours.
Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna - Clone 5 (Crustacea, Daphniidae)
Origin - German Environmental Ministry
Pre-treatment - the animals are cultivated in a climate controlled room
(light/dark = 14h / 10h; illumination: GroLux fluorescent tubes).
The animals were fed with algae (Desmodesmus subspicatus).
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d
Test temperature:
19.7 to 20.8 deg C
pH:
8.0 to 8.2
Dissolved oxygen:
8.3 to 8.8 mg/l
Salinity:
freshwater
Nominal and measured concentrations:
Nominal concentrations were: 0, 2500, 5000 mg/l.

Concentration analysis (TOC) of the control and 2500 and 5000 mg/l test concentrations gave results of <10 mg/l. Indicating very low solubility of the test substance.
Details on test conditions:
40 animals/conc. (10 each per 4 replicates)
400 ml of solution in 800 ml glass beakers.
14h light / 10h dark - GroLux fluorescent tubes.
Animals were fed daily with algae (Desmodesmus subspicatus)
Reference substance (positive control):
no
Duration:
21 d
Dose descriptor:
EL50
Effect conc.:
> 5 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
immobilisation
Remarks:
Parental generation
Duration:
21 d
Dose descriptor:
EL50
Effect conc.:
< 2 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
NOELR
Effect conc.:
< 2 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Key result
Duration:
21 d
Dose descriptor:
EL50
Effect conc.:
ca. 1 400 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: inhibition of reproduction
Key result
Duration:
21 d
Dose descriptor:
NOELR
Effect conc.:
ca. 17 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: inhibition of reproduction
Details on results:
Immobilisation of Parental Generation: (at end of test)
Control - 10%
2500 mg/l - 20%
5000 mg/l - 28%

Date of first appearance of young (F1):
Control - 8d
2500 mg/l - 10d
5000 mg/l - 12d

Number of living young produced (at end of test):
Control - 712
2500 mg/l - 265 (62.8% inhibition of reproduction vs. control)
5000 mg/l - 177 (75.1% inhibition of reproduction vs. control)

Number of dead young produced (at end of test):
Control - 10
2500 mg/l - 16
5000 mg/l - 27
Reported statistics and error estimates:
Graphic evaluation on probability paper gave approximate results of the inhibition of reproduction.
Validity criteria fulfilled:
yes
Conclusions:
The chronic toxicity of Hydrocarbons, C11 -C20, n-alkanes, isoalkanes, cyclics, aromatics (2 -30%) as measured by reproductive success to the water flea (Daphnia magna) was evaluated in freshwater. Hydrocarbons, C11 -C20, n-alkanes, isoalkanes, cyclics, aromatics (2 -30%) showed toxic effects as a Water Accommodated Fraction (WAF) with a 21 -day EL50 of approximately 1400 mg/l and an NOELR of 17 mg/l (based on inhibition of reproduction).
Executive summary:

The chronic toxicity of Hydrocarbons, C11 -C20, n-alkanes, isoalkanes, cyclics, aromatics (2 -30%) as measured by reproductive success to the water flea (Daphnia magna) was evaluated in freshwater. Hydrocarbons, C11 -C20, n-alkanes, isoalkanes, cyclics, aromatics (2 -30%) showed toxic effects as a Water Accommodated Fraction (WAF) with a 21 -day EL50 of approximately 1400 mg/l and an NOELR of 17 mg/l (based on inhibition of reproduction).

Description of key information

There is no data available for this substance. However, key data is available for structural analogues Hydrocarbons, C11 -C20, n-alkanes, isoalkanes, cyclics, aromatics (2 -30%). The data is read across to this substance based on analogue read across and a discussion and report on the read across strategy is provided as an attachment in IUCLID Section 13. Key information is summarised below:

Hydrocarbons, C11 -C20, n-alkanes, isoalkanes, cyclics, aromatics (2 -30%) showed toxic effects as a Water Accommodated Fraction (WAF) with a 21 -day EL50 of approximately 1400 mg/l and an NOELR of 17 mg/l (based on inhibition of reproduction).

Key value for chemical safety assessment

Additional information

One study was available and input as an endpoint record. The study is summarised below.

The study from Haltermann (2003a) examined the long-term toxicity (as measured by reproductive success) of the test substance Hydrocarbons C11 -C20, n-alkanes, isoalkanes, cyclics, aromatics (2 -30%) to Daphnia magna in freshwater. Hydrocarbons, C11 -C20, n-alkanes, isoalkanes, cyclics, aromatics (2 -30%) showed toxic effects as a Water Accommodated Fraction (WAF) with a 21 -day EL50 of approximately 1400 mg/l and an NOELR of 17 mg/l (based on inhibition of reproduction).