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Acute Fish Toxicity:

Water accommodated fractions of hydrocarbons, C16-C20, n-alkanes, isoalkanes, cyclics, aromatics (2-30%) did not cause acute toxicity to fish with rainbow trout, Oncorhynchus mykiss at a nominal loading of 1000 mg/l. These data are used as read-across data to hydrocarbons, C14-C18, n-alkanes, isoalkanes, cyclics, aromatics (2 -30%).

Chronic Fish Toxicity:

Hydrocarbons, C16-C20, n-alkanes, isoalkanes, cyclics, aromatics (2-30%) did not cause toxicity to fish in a 21-day toxicity study with Brachydanio rerio following standard test guidelines at a nominal loading of 5000 mg/l. These results indicate that hydrocarbons, C16-C20, n-alkanes, isoalkanes, cyclics, aromatics (2-30%) is not expected to be chronically toxic to fish. These data are used as read-across data to hydrocarbons, C14-C18, n-alkanes, isoalkanes, cyclics, aromatics (2-30%).

Acute Invertebrate Toxicity:

Water accommodated fractions of hydrocarbons, C16-C20, n-alkanes, isoalkanes, cyclics, aromatics (2-30%) did not cause acute toxicity to the freshwater invertebrate, Daphnia magna at a nominal loading of 1000 mg/l. These data are used as read-across data to hydrocarbons, C14-C18, n-alkanes, isoalkanes, cyclics, aromatics (2-30%).

Chronic Invertebrate Toxicity:

Hydrocarbons, C16-C20, n-alkanes, isoalkanes, cyclics, aromatics (2-30%) did not cause toxicity in a 21-day toxicity study with Daphnia magna following standard test guidelines at a nominal loading of 1400 mg/l, with a NOELR of 17 mg/l (based on inhibition of reproduction). These results indicate that hydrocarbons, C16-C20, n-alkanes, isoalkanes, cyclics, aromatics (2-30%)is not expected to be chronically toxic to freshwater invertebrates. These data are used as read-across data to hydrocarbons, C14-C18, n-alkanes, isoalkanes, cyclics, aromatics (2-30%).

Algal Toxicity:

Water accommodated fractions of hydrocarbons, C16-C20, n-alkanes, isoalkanes, cyclics, aromatics (2-30%) did not cause acute toxicity to the freshwater green algae, Pseudokirchneriella subcapitata, at a nominal loading of 1000 mg/l. These data are used as read-across data to hydrocarbons, C14-C18, n-alkanes, isoalkanes, cyclics, aromatics (2-30%).

Microorganisms Toxicity:

Water Accomodated Fractions of hydrocarbons, C16-C20, n-alkanes, isoalkanes, cyclics, aromatics (2-30%), did not inhibit microbial respiration in a sludge sample at a concentration of 1000 mg/l. Therefore, Hydrocarbons, C16-C20, n-alkanes, isoalkanes, cyclics, aromatics (2-30%), is not expected to adversely affect microbial activity in a wastewater treatment plant. These data are used as read-across data to hydrocarbons, C14-C18, n-alkanes, isoalkanes, cyclics, aromatics (2-30%).