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Toxicity to microorganisms

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Description of key information

Based on the analogue approach justification, the following results on the toxicity to microorganisms obtained on Vinasses, residue of fermentation, can be applied to the Vinasses, residue of fermentation, depotassified  :
EC50 (3h) > 100 mg/L

Key value for chemical safety assessment

Additional information

One key study is available on Vinasses, residue of fermentation (Desmares-Koopmans, 2010). This study was performed according to the OECD guideline 209 “Activated Sludge, Respiration Inhibition Test” using activated sludge of a predominantly domestic sewage. No inhibition of the respiration rate was observed, resulting in an EC50 (3 h) of > 100 mg/L (based on nominal concentrations). The observation of no toxic properties of the test substance Vinasses, residue of fermentation is underlined by the absence of inhibiting effects in the toxicity control in an OECD 301B test (see technical dossier chapter 5.2.1). Therefore, a disturbance in the biodegradation process of sewage treatment plant is not anticipated.

Vinasses, residue of fermentation and vinasses, residue of fermentation, depotassified contain very similar dry matter contents and the same types of components with similar ranges.The test results obtained for Vinasses, residue of fermentation on biodegradation, with fish (short term), daphnia (long term), algae and activated sludge reveal no persistency and no toxicity hazard for the aquatic environment. Therefore, based on the analogue justification approach,an analogy can be done on these properties between Vinasses, residue of fermentation and Vinasses residue of fermentation, depotassified.


Thus, Vinasses, residue of fermentation, depotassified, is considered to be not toxic to waste water (activated sludge) bacteria at a nominal concentration of 100 mg/L.