3,4-dichlorobut-1-ene

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data from peer-reviewed handbook or collection of data

Data source

Reference

Reference 1

Reference Type:

review article or handbook

Title:

Unnamed

Year:

1992

Reference 2

Reference Type:

secondary source

Title:

3,4-Dichlorobut-1-ene - CAS No: 760-23-6

Author:

OECD SIDS

Year:

2003

Bibliographic source:

SIDS Initial Assessment Report for 11th SIAM, UNEP Publications

Reference 3

Reference Type:

secondary source

Title:

Chemical Risk Information Platform- Physical Chemical data

Author:

NITE

Year:

2010

Bibliographic source:

http://www.safe.nite.go.jp/data/hazkizon/pk_e_kizon_disp.html?k_no=0018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: "Bioaccumulation test of chemical cubstance in fish and shellfish", order of the prime minister, the minister of health and welfare, the minister of international trade and industry No 1, Japan, 1974

Principles of method if other than guideline:

This guideline corresponds to OECD Guideline 305C (Bioaccumulation: Test for the degree of bioconcentration in Fish), May 12, 1981.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

3,4-dichlorobut-1-ene

EC Number:

212-079-0

EC Name:

3,4-dichlorobut-1-ene

Cas Number:

760-23-6

Molecular formula:

C4H6Cl2

IUPAC Name:

3,4-dichlorobut-1-ene

Details on test material:

purity not specified

Radiolabelling:

no

Test organisms

Test organisms (species):

Cyprinus carpio

Details on test organisms:

Supplier: Sugishima fish farm, Japan
Weight: about 30g, Length: about 10 cm, Lipid content: 2-6 % (at the initiation of exposure)

Study design

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

42 d

Test conditions

Test temperature:

25+/-2 °C

Dissolved oxygen:

6-8 mg/L

Details on test conditions:

Test tank: 100L glass tank
Number of fish: 15-20 fish/Level
Renewal rate of test solutions: flow rate 200-800 mL/min

Nominal and measured concentrations:

Considering of the result of preliminary test for 48-hr LC50 and analytically detecting limit of the test substance, test concentrations were decided.
1st concentration: 0.26 mg/L
2nd concentration:0.026 mg/L

Results and discussion

Lipid content

Lipid content:

> 2 - < 6 %

Time point:

start of exposure

Bioaccumulation factor

Bioaccumulation factor 1

Type:

BCF

Value:

0.59 - 2.11 dimensionless

Basis:

whole body w.w.

Calculation basis:

steady state

Remarks on result:

other: Conc.in environment / dose:0.26 mg/L

Bioaccumulation factor 2

Type:

BCF

Value:

0.28 - < 13.34 dimensionless

Basis:

whole body w.w.

Calculation basis:

steady state

Remarks on result:

other: Conc.in environment / dose:0.026 mg/L

Details on results:

Preliminary test:
species: Rice fish (Oryzias latipes)
48h-LC50: 22.6 mg/L

Any other information on results incl. tables

Calculation of bioconcentration factor

BCF = concentration of test substance in fish/concentration of test substance in water