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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: No GLP and method similar to OECD 471.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1983
Report Date:
1983

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
Deviations:
no
GLP compliance:
no
Type of assay:
bacterial reverse mutation assay

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:

- Name of test material (as cited in study report): Monochloressigsäuremethylester
- Physical state: Colourless liquid
- Analytical purity: 99.4%
- Lot/batch No.: KW 0727267-5 vom 28.10.82
- Storage condition of test material: Dark at 20ºC

Method

Species / strain
Species / strain:
other: Salmonella typhimurium TA 100, TA 98, TA 1535, TA 1537, TA 1538 and Escherichia coli WP2uvrA
Metabolic activation:
with and without
Metabolic activation system:
S-9 mix
Test concentrations with justification for top dose:
0, 4, 20, 100, 500, 2500 and 5000 µg/plate (dissolved in 100 µl DMSO)
Vehicle:
- Vehicle(s)/solvent(s) used: DMSO
Controlsopen allclose all
Solvent controls:
yes
Positive controls:
yes
Positive control substance:
sodium azide
Remarks:
without metabolic activation: TA100 and TA1535
Positive controls:
yes
Positive control substance:
9-aminoacridine
Remarks:
without metabolic activation: TA1537
Positive controls:
yes
Positive control substance:
2-nitrofluorene
Remarks:
without metabolic activation: TA98 and TA1538
Positive controls:
yes
Positive control substance:
other: N-methyl-N´-nitro-N-nitrosoguanidine
Remarks:
without metabolic activation: WP2uvrA
Positive controls:
yes
Positive control substance:
benzo(a)pyrene
Remarks:
with metabolic activation: TA98, TA100, TA1535, TA1537, TA1538 and WP2uvrA
Positive controls:
yes
Positive control substance:
other: 2-aminoanthracene
Remarks:
with metabolic activation: TA98, TA100, TA1535, TA1537, TA1538 and WP2uvrA
Details on test system and conditions:

METHOD OF APPLICATION: in agar (plate incorporation);

DURATION
- Exposure duration: 48 to 72 hours at 37 ºC in the dark

NUMBER OF REPLICATIONS: Triplicates

DETERMINATION OF CYTOTOXICITY
- Method: Thinning of the bacterial lawn and reduction on the number of colonies. The solvent control is compared with the number of colonies per plate in the presence of the test compound (surviving factor).

Results and discussion

Test results
Species / strain:
other: Salmonella typhimurium TA98, TA100, TA1535, TA1537, TA1538 and E. coli WP2uvrA
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity:
yes
Remarks:
At doses of 2500 µg/plate
Vehicle controls valid:
yes
Positive controls valid:
yes
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

The test compound did not cause a significant increase in the number of revertant colonies with any of the tester strains neither in the absence nor presence of S9-mix. It is concluded that the test substance is not mutagenic in this bacterial test system neither in the absence nor in the presence of metabolic activation.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative

The test compound did not cause a significant increase in the number of revertant colonies with any of the tester strains neither in the absence nor presence of S9-mix. It is concluded that the test substance is not mutagenic in this bacterial test system neither in the absence nor in the presence of metabolic activation.
Executive summary:

The test substance methyl chloroacetate was tested for mutagenicity with the strains TA98, TA100, TA1535, TA1537 and TA1538 of Salmonella typhimurium and WP2uvrA of E.coli. The mutagenicity studies were performed in the absence and in the presence of metabolic activation. A dose range of six concentrations from 4 µg/plate to 5000 µg/plate was used. Control plates and positive controls were also included in the study. The test compound did not cause a significant increase in the number of revertant colonies with any of the tester strains neither in the absence nor presence of S9-mix. It is concluded that the test substance is not mutagenic in this bacterial test system neither in the absence nor in the presence of metabolic activation.