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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: No guideline was followed and no GLP. However, the test method was similar to OECD 402.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1979
Report Date:
1979

Materials and methods

Principles of method if other than guideline:
No guideline was followed but the test method was similar to OECD 402.
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): monochloressigsäuremethylester
- Physical state: colourless liquid

Test animals

Species:
rat
Strain:
Wistar
Sex:
female
Details on test animals and environmental conditions:
TEST ANIMALS
- Source: own breeding
- Weight at study initiation: 199 ± 9.93 g
- Diet (e.g. ad libitum): Altromin 1324 (Altromin GmbH, Lage/Lippe), ad libitum
- Water (e.g. ad libitum): tap water, ad libitum

Administration / exposure

Type of coverage:
occlusive
Vehicle:
unchanged (no vehicle)
Details on dermal exposure:
TEST SITE
- Area of exposure: ca. 30 cm2
- Type of wrap if used: aluminium foil (6 x 8 cm) wrapped by an elastic plaster (Elastoplast, 8 cm wide)


REMOVAL OF TEST SUBSTANCE
- Washing (if done): with water
- Time after start of exposure: 24 hours


Duration of exposure:
24 hours
Doses:
50, 100, 125, 160, 315 and 500 mg/kg bw
No. of animals per sex per dose:
Six females per dose
Control animals:
not required
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: body weight was recorded at 0, 7 and 14 days
- Other examinations performed: clinical signs, necropsy.
Statistics:
Probit analysis

Results and discussion

Effect levels
Sex:
female
Dose descriptor:
LD50
Effect level:
136.6 mg/kg bw
95% CL:
121.3 - 153.7
Mortality:
Mortality was observed at 125, 160, 315 and 500 mg/kg bw.
Clinical signs:
Prone position, passivity, irregular and laboured respiration, squatting position, piloerection, dizziness, impared balance, hyporeflexia and increased red coloured lacrimation.
Gross pathology:
Animals showed clearly extended blood vessels in the subcutaneous area of the treated skin.

Any other information on results incl. tables

Table 7.2.3 -1: Mortality

Dose (mg/kg bw)

Mortality

50

0/6

100

0/6

125

2/6

160

5/6

315

6/6

500

6/6

Applicant's summary and conclusion

Interpretation of results:
Toxicity Category II
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
The dermal LD50 was 136.6 mg/kg bw with a 95% confidence interval between 121.3 and 153.7 mg/kg bw.
Executive summary:

The test substance methyl chloroacetate was administered dermally to groups of female Wistar rats at the doses of 50, 100, 125, 160, 315 and 500 mg/kg bw during 24 hours. Animals were subjected to daily observations and weekly determinations of body weight. Macroscopic examination was performed in the animals. Mortality was observed at 125, 160, 315 and 500 mg/kg bw. The dermal LD50 was 136.6 mg/kg bw with a 95% confidence interval between 121.3 and 153.7 mg/kg bw.