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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: OECD guideline and GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report Date:
1988

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method B.2 (Acute Toxicity (Inhalation))
Deviations:
no
GLP compliance:
yes
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): monochloressigsäuremethylester
- Analytical purity: 99%

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female
Details on test animals and environmental conditions:
TEST ANIMALS
- Source: Hoechst AG, Kastengrund, SPF-Zucht
- Age at study initiation: 8-10 months
- Weight at study initiation: males: 203-225 g; females: 191-220 g
- Diet (e.g. ad libitum): Altromin 1324 (Altromin-GmbH, Lage/Lippe), ad libitum
- Water (e.g. ad libitum): tap water, ad libitum


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 2 ºC
- Humidity (%): 50 ± 20%
- Photoperiod: 12 hours light/12 hours dark

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
other: unchanged (no vehicle)
Details on inhalation exposure:
The test substance was applied continuously with a permanent drip apparatus and was evaporated at 130 ºC.
Analytical verification of test atmosphere concentrations:
yes
Duration of exposure:
4 h
Concentrations:
90, 210, 315 and 385 ppm
No. of animals per sex per dose:
5 animals per sex per dose
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: two daily observations and body weights were recorded at 0, 7 and 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, necropsy.

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LC50
Effect level:
210 - 315 ppm
Exp. duration:
4 h
Mortality:
Mortality was observed at 315 and 385 ppm.
Clinical signs:
90 ppm: squatting position, uncoordinated walk, irregular and laboured respiration.
At the higher dose groups: prone position, reeling walk, noisy respiration, hyporeflexia, corneal opacity and cyanose.
Gross pathology:
Dark red dyed lungs. While cutting the lung, foamy liquid escaped in the majority of the animals. The intestine mucous membrane was red and was filled with foamy liquid.

Any other information on results incl. tables

Table 7.2.2 -2: Mortality

Concentration (ppm)

Mortality

Males

Females

90

0/5

0/5

210

0/5

0/5

315

3/5

4/5

385

5/5

5/5

Applicant's summary and conclusion

Interpretation of results:
Toxicity Category II
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
The 4-h LC50 in rats was determined to be between 210 and 315 ppm (638 and 957 mg/m3).
Executive summary:

An acute inhalation toxicity study was carried out with the substance methyl chloroacetate. Groups of five Wistar rats per sex were exposed at 90, 210, 315 and 385 ppm for 4 hours. The 4-h LC50 in rats was determined to be between 210 and 315 ppm (638 and 957 mg/m3).