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Diss Factsheets

Administrative data

Endpoint:
long-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Only secondary literature

Data source

Reference
Reference Type:
review article or handbook
Title:
European Union Risk Assessment Report - o-Anisidin
Author:
European Chemicals Bureau
Year:
2002
Bibliographic source:
European Commission, Joint Research Center, EUR 19834 EN, 2nd Priority list, Volume 15

Materials and methods

Principles of method if other than guideline:
Acute toxicity tests: duration 4-14 days; static (14d), flow-through (14d) and semi-static (4d);

Test material

Constituent 1
Chemical structure
Reference substance name:
o-anisidine
EC Number:
201-963-1
EC Name:
o-anisidine
Cas Number:
90-04-0
Molecular formula:
C7H9NO
IUPAC Name:
2-methoxyaniline
Details on test material:
Commercially available o-anisidine with a purity of >=99%;

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
other: Poecilia reticulata, Oryzias latipes

Study design

Test type:
other: static (14d), flow-through (14d) and semi-static (4d)
Water media type:
freshwater
Limit test:
no
Remarks on exposure duration:
4-14

Results and discussion

Effect concentrationsopen allclose all
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
18 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: static, Poecilia reticulata
Duration:
14 d
Dose descriptor:
EC50
Effect conc.:
18 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
behaviour
Remarks on result:
other: static, Poecilia reticulata
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
not specified
Remarks on result:
other: Oryzias latipes, flow-through
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Oryzias latipes, flow-through
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
196 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Oryzias latipes, flow-through

Applicant's summary and conclusion

Validity criteria fulfilled:
not applicable
Conclusions:
In different toxicity tests on the fish species Poecilia reticulata and Oryzias latipes the following effect concentrations were determined for the submission substance:
- Poecilia reticulata, 14d, static: EC50 = 18 mg/L; LC50 = 165 mg/L;
- Oryzias latipes: a) 14 d, flow through: NOEC = 25 mg/L, LC50 >100 mg/L; b) 4d, semi-static: LC50 = 196 mgL;
Executive summary:

In different toxicity tests on the fish species Poecilia reticulata and Oryzias latipes the following effect concentrations were determined for the submission substance:

Poecilia reticulata, 14d, static: EC50 = 18 mg/L; LC50 = 165 mg/L; -

Oryzias latipes: a) 14 d, flow through: NOEC = 25 mg/L, LC50 >100 mg/L; b) 4d, semi-static: LC50 = 196 mgL;