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No short-term terrestrial test data is available for diamines. One long-term terrestrial test is performed with diamines.

The effects of N-tallow alkyltrimethylenediamines on mortality, biomass and reproduction of Eisenia fetida was tested according to OECD 222 under a static exposure for 28 days. Natural soil (Lufa 2.2) was used as substrate, and the different concentration of the substance mixed with the substrate. The concentrations applied were 125, 250, 500, and 1,000 mg/kg soil dry weight. In addition tests using control and vehicle control were performed.

The test is valid without restrictions and the results are reliable. After 28 days of exposure, no effects on survival of the adult worms were observed in all concentrations. After the following four weeks, no significant effects on the reproduction rate (average number of juveniles) were observed compared to the control. Hence, the NOEC is 1000 mg/kg dw soil.