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Short-term toxicity to fish

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Description of key information

The test substance is with high probability acutely not harmful to fish.

Key value for chemical safety assessment

Additional information

Under supervision of the Japanese Ministry of the Environment an acute fish test according to OECD 203 was conducted under GLP and published in 2000. The test species was Oryzias latipes. The fish were exposed for 96 hours under semi-static conditions to nominal test concentrations of 9.5 to 100 mg/L diethylamine. The test concentrations were analytically verified.

The test solutions without pH-adjustment showed initial pH values between 9.1 and 10.6. The acceptable pH range for fish given in the OECD TG 203 (6.0 to 8.5) was only kept in the control, while the test solutions showed initial pH values above 8.5. Therefore, the highest test concentration (100 mg/L) was also tested with neutralised pH value. A 96 -h LC50 was determined for non-neutralised samples to be 26.7 mg/L (95% CL: 19.7 - 34.8 mg/L, nominal). In the neutralised highest test concentration of 100 mg/L no fish died during the 96 -h of exposure (96 -h LC50 > 100 mg/L, for neutralised test solutions, NITE, 2000, report no.: NMMP/E99/4070).

These results are supported by several further publications. Calamari and colleagues, 1980, studied the effect of the test substance on Oncorhynchus mykiss using an Italian standard test guideline. They also investigated the effect of water hardness on the toxic effects of diethylamine. The LC50 values were determined to be 182 and 25 mg/L in hard and soft water, respectively. It is commonly accepted by European Regulatory Authorities, that within the European Union, the water hardness is rather high, therefore the LC50 determined in soft water is of low relevance for the risk assessment. It can be assumed that the hard water buffered the pH, while the pH in the test solution prepared in soft water were high. It can be concluded that pH effects induced by diethylamine are not relevant in the hard water test. The higher LC50 value is supported by several publications determining LC50 values far higher than the one published for the exposure of Oncorhynchus mykiss in soft water (855 and 130mg/L, respectively; Brooke et al., 1984; van Leeuwen et al., 1985).

Therefore, it was concluded that diethylamine is with high probability acutely not harmful to fish.