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Diss Factsheets

Ecotoxicological information

Toxicity to terrestrial plants

Currently viewing:

Administrative data

Endpoint:
toxicity to terrestrial plants: short-term
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: unsuitable test system: agar solution was used instead soils.

Data source

Reference
Reference Type:
publication
Title:
Study on the Development of the Test Methods for Evaluation of the Effects of Chemicals on Plants
Author:
Hikino H
Year:
1979
Bibliographic source:
Environment Agency Japan

Materials and methods

Principles of method if other than guideline:
According Y. Yamaguchi and S. Tsunoda , Japan Breeding, 19, 350 (1969)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Benzothiazole-2-thiol
EC Number:
205-736-8
EC Name:
Benzothiazole-2-thiol
Cas Number:
149-30-4
Molecular formula:
C7H5NS2
IUPAC Name:
1,3-benzothiazole-2-thiol
Details on test material:
purity not specified

Sampling and analysis

Analytical monitoring:
not specified

Test substrate

Details on preparation and application of test substrate:
An agar agar solution (0.6 %), sterilised at 120 °C for 30 min, cooled down to room temperature was used for the test.

Study design

Study type:
laboratory study

Test conditions

Test temperature:
29°C for rice
25°C for turnip and soybean
Details on test conditions:
Six groups (50 each) of rice, turnip or soybean seeds were placed on the agar medium and kept in the dark in an incubator at 29 °C for rice or at 25 °C for turnip and soybean.

Results and discussion

Effect concentrationsopen allclose all
Species:
Oryza sativa
Duration:
5 d
Dose descriptor:
other: 50 % root length
Effect conc.:
100 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Oryza sativa
Duration:
5 d
Dose descriptor:
other: 89 % leaf length
Effect conc.:
100 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Oryza sativa
Duration:
5 d
Dose descriptor:
NOEC
Effect conc.:
10 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Brassica rapa
Duration:
4 d
Dose descriptor:
other: 42 % root length
Effect conc.:
100 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Brassica rapa
Duration:
4 d
Dose descriptor:
other: 84 % leaf length
Effect conc.:
100 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Brassica rapa
Duration:
4 d
Dose descriptor:
NOEC
Effect conc.:
10 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Glycine max (G. soja)
Duration:
4 d
Dose descriptor:
other: 62 % root length
Effect conc.:
100 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Glycine max (G. soja)
Duration:
4 d
Dose descriptor:
other: 77 % leaf length
Effect conc.:
100 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Glycine max (G. soja)
Duration:
4 d
Dose descriptor:
other: 72 % root length
Effect conc.:
1 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Glycine max (G. soja)
Duration:
4 d
Dose descriptor:
other: 82 % leaf length
Effect conc.:
1 other: ppm
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Details on results:
Concentrations of 1, 10, and 100 ppm were tested.

Applicant's summary and conclusion

Executive summary:

The development of roots and leafs on rice, turnip and soybean was examined on an agar solution, and compared to the growth in the controls. For rice a 5-day NOEC of 10 mg/l was determined, and for turnip a 4-day NOEC of 10 mg/l was received. On soybean a growth of 72 % was obtained for root length, and 82 % growth for leaf length within 4 days at a test concentration of 1 mg/l.