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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report Date:
1995

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: OECD 202, part 2, draft 6/5/91: "Prolonged Toxicity study with Daphnia magna: Effects on reproduction "
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Name of test material: Alkansulfonate -Na
- Analytical purity: 61 % related on total sulfonate (56 % related on monosulfonate)
- Batch No. E 11 A 03673

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Sampling method: For the analysis of the test substance concentrations, a sample was taken from freshly prepared stock solution (nominal 100 mg/L) and dublicate samples were taken from the freshly prepared test media of the nominal test concentrations 0.32, 1.0 and 3.2 mg/L and the control at the first treatment period (day 0), at a treatment period in the second week (day 12) and at a treatment period in the last week (day 16). For the determination of the stability of the test substance under the test conditions, additional samples were taken at the end of two treatment periods (on day 14 and 19). All samples samples were taken out of the actual test (including the algae food).

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was homogenized by intesive stirring. Then an adequate amount of the homogenized test substance was weighted into test water for the preparation of a stock solution (100 mg/L). The test concentrations were added to the test water without any further pretreatment. The test media were intensively stirred before introduction of daphnia.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Strain/clone: N.a.
- Source: Clone was supplied by the BASF AG in January 1993, and then was bred in the laboratories of RCC under the same conditions as used in the test.
- Feeding during test: Yes
- Food type: Green algae (Scenedesmus subspicatus)
- Frequency: Day 0, 1, 2, 5, 6, 7, 9, 12, 13, 14, 15, 16, 19, 20 and 21.

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
22 d

Test conditions

Hardness:
250 mg/L CaCO3
Test temperature:
20.5-21.2 °C
pH:
7.7-8.7
Dissolved oxygen:
8.4-10.6 mg/L
Nominal and measured concentrations:
Nominal concentrations: control, 0.1, 0.32, 1.0, 3.2 and 10.0 mg/L.
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: glass beaker, 100 ml containing 80 ml test medium.
- Aeration: no
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4-medium
- Culture medium different from test medium: No
- Intervals of water quality measurement: at the beginning and end of each treatment period in the lowest, highest and control treatment.

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16/8 hours light/dark cycle
- Light intensity: approximately 370-600 lux

EFFECT PARAMETERS MEASURED: Reproduction and mortality

Results and discussion

Effect concentrationsopen allclose all
Duration:
22 d
Dose descriptor:
NOEC
Effect conc.:
0.36 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
act. ingr.
Basis for effect:
reproduction
Remarks on result:
other: 61% a.i. in test mat.
Duration:
22 d
Dose descriptor:
NOEC
Effect conc.:
1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
22 h
Dose descriptor:
NOEC
Effect conc.:
0.6 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
22 d
Dose descriptor:
LOEC
Effect conc.:
3.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
22 d
Dose descriptor:
LOEC
Effect conc.:
1.6 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
reproduction

Any other information on results incl. tables

Except for the reported mortality, no particular signs of toxicity were observed at the test animals during the test.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Executive summary:

The influence of the test substance on the reproduction and survival rate of Daphnia magna was investigated in a 22-day semi-static test following the proposal for an update of OECD guideline 202, part II, draft 6/5/91. The test was performed in compliance with the GLP.

The nominal concentrations tested were 0.10, 0.32, 1.0, 3.2 and 10.0 mg/L and a control.

Analytical results:

The analytically determined test substance concentrations in the freshly prepared test media varied in the range from 75 to 108 % of nominal, in the mean over all measurements from 92 to 102 % of nominal.

During the test medium renewal periods of 48 to 72 hours, the test substance concentrations strongly decreased. In the samples, taken out of the test media of the biological test (including algal food) after 48 hours respectively 72 hours exposure periods, concentrations at or below the determination limit of the analytical method were determined. The arithmetic mean values between the measured test substance concentrations in the freshly prepared test media and the measured concentrations in the 48 hours respectively 72 hours old test media amounted to 0.18 mg/L (= 55 % of nominal) at nominally 0.32 mg/L, to 0.6 mg/L (61 % of nominal) at nominally 1.0 mg/L, and to 1.9 mg/L (55 % of nominal) at nominally 3.2 mg/L.

Biological test results:

Based on the survival rates and the reproduction rates of the test animals, the highest concentration tested without toxic effects (NOEC) after the exposure period of 22 days was determined to be 1.0 mg/L (nominal; mean measured: 0.6 mg/L). The lowest concentration tested with toxic effects (LOEC) was nominally 3.2 mg/L (mean measured: 1.9 mg/L) due to the high mortality rate of Daphnia magna at this test concentration.