Registration Dossier
Registration Dossier
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EC number: 265-191-7 | CAS number: 64742-88-7 A complex combination of hydrocarbons obtained from the distillation of crude oil or natural gasoline. It consists predominantly of saturated hydrocarbons having carbon numbers predominantly in the range of C9 through C12 and boiling in the range of approximately 140°C to 220°C (284°F to 428°F).
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- genetic toxicity in vitro
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study was classified as reliable without restriction because it is an acceptable and a well-documented study report.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
Materials and methods
- Principles of method if other than guideline:
- The method used for this study was a modified Ames test (ASTM E1687). Because the standard Ames test is considered to be inappropriate for testing the mutagenicity of water insoluble petroleum products, the Ames test was modified as described in Blackburn and co-workers (1986). Substances tested were considered positive if they showed a mutagenicity index (MI) greater than 1.0.
- GLP compliance:
- not specified
- Type of assay:
- other: Modified Ames assay
Test material
- Reference substance name:
- Hydrodesulfurised kerosine (CAS# 64742-81-0)
- IUPAC Name:
- Hydrodesulfurised kerosine (CAS# 64742-81-0)
- Details on test material:
- IUCLID4 Test substance: other TS: Kerosine, CAS No. 64742-81-0
Three samples were tested.
Sample CAS No. Treatment Source Crude
1 64742-81-0 hydrotreated North Sea
1 64742-81-0 hydrotreated Middle East
- Name of test material (as cited in study report): DMSO Extracts of kerosine
- Storage condition of test material: indoors under nitrogen blanket.
- Other: Temperature of storage space maintained above 0 degrees Celsius
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: S. typhimurium TA98
- Metabolic activation:
- not specified
- Test concentrations with justification for top dose:
- 50 µl/mL
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- not specified
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Remarks on result:
- other: strain/cell type: S. typhimurium TA98
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
The mutagenicity indices of the three samples were all zero, thus demonstrating an absence of mutagenic activity. Interestingly, none of the samples was found to contain any measurable 3-7 ring PACs.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
None of the kerosines tested were mutagenic. - Executive summary:
A standard Ames test was conducted on wet treated, straight-run kerosines and hydrotreated kerosines. S. typhimurium were exposed to kerosine and solvent DMSO at concentrations of 50 µL/mL. Because the standard Ames test is considered inappropriate for testing the mutagenicity of water insoluble petroleum products, the Ames test was modified according to Blackburn (1986) in order to be applied to straight-run kerosines. Substances tested were considered positive if they showed a mutagenicity index greater than 1.0. All three kerosines tested had MI values of 0.0.
This study received a Klimisch score of 1 and is classified as reliable without restrictions because it is an acceptable and a well-documented study report.
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