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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: scientifically acceptable and sufficinet documented

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1972

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 402 (Acute Dermal Toxicity)
Principles of method if other than guideline:
Undiluted 3-chloro-p-toluidine was applied on the shaved belly skin of rats for 4 hours in doses of 100 to 1000 µl/kg (117 to 1170 mg/kg). After 4 hours the substance was removed with water and soap. The animals were observed for 7 days.
GLP compliance:
not specified
Test type:
standard acute method

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Test material form:
other: liquid
Details on test material:
technically pure, undiluted 3-chloro-p-toluidine

Test animals

Species:
rat
Strain:
Wistar
Sex:
male

Administration / exposure

Type of coverage:
not specified
Vehicle:
unchanged (no vehicle)
Duration of exposure:
4 hours
Doses:
100 µl (= 117 mg)/kg bw, 250 µl (= 292.5 mg/kg bw), 500 µl (= 585 mg/kg bw), 600 µl (= 702 mg/kg bw), 800 µl (= 936 mg/kg bw), 1000 µl (= 1170 mg/kg bw).
No. of animals per sex per dose:
5 rats (100 µl), 5 rats (250 µl), 10 rats (500 µl), 10 rats (600 µl), 10 rats (800 µl), 5 rats (1000 µl).
Control animals:
no

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
LD50
Effect level:
ca. 765 mg/kg bw
Based on:
test mat.

Any other information on results incl. tables

From 2 to 6 hours after application of the test substance,typical signs of intoxication (cyanosis, laboured breathing, sedation) were observed. The highest dose without findings was 100 µl (117 mg/kg bw) and the lowest lethal dose was 600 µl (702 mg/kg bw).

Applicant's summary and conclusion

Executive summary:

Undiluted 3-chloro-p-toluidine was applied on the shaved belly skin of rats for 4 hours in doses of 100 to 1000 µl/kg (117 to 1170 mg/kg bw). After 4 hours the substance was removed with water and soap. The animals were observed for 7 days.

LD50 (4 hours exposure) = 650 µl 3-chloro-p-toluidine/kg bw (= ca. 765 mg/kg bw).