3-chloro-p-toluidine

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1985

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions.

Principles of method if other than guideline:

The method described in the UBA test protocol: Letale Wirkung beim Zebrabärbling Brachydanio rerio, (LC 0, LC 50, LC 100; 48 - 96 h) Stand Mai 1984, which is comparable to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test', assesses the acute toxic effects (mortality) of various concentrations of a test item to a freshwater fish species.
The purpose of this method was to determine that concentration which causes a 50 % mortality (= LC 50) or, if conducted as a limit test, to determine the acute toxic effects at a maximum test concentration of 100 mg/L or at the limit of water solubility, respectively.
In the main test, fish are exposed to the test item added to water at a range of concentrations for a period of 96 hours. Defined concentrations of the test item will lead to a certain percentage of dead fish at the end of the 96 hour study period. Mortalities are recorded at 24-hour intervals. The LC 0 and the LC 100 are determined directly from the test results. During the test, a temperature range of 20 - 24 °C is to be maintained in the test vessels, with a maximum temperature fluctuation of +/- 1 °C in each individual test. PH and oxygen values are measured at the beginning of the test and every 24 hours thereafter.
The test is conducted as static test in which no flow of test solution occurs and solutions remain unchanged throughout the duration of the test.
The dissolved oxygen concentration should be above 60 % of the air-saturated value throughout the study.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

not specified

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

20 - 24 °C

Dissolved oxygen:

> 5 mg/L

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

17.8 mg/L

Duration:

96 h

Dose descriptor:

other: LC20

Effect conc.:

23.7 mg/L

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

24.8 mg/L

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

31 mg/L

Executive summary:

3 -chlor-p-toluidine was measured to cause LD50 = 24.8 mg/L using Danio rerio in a 96 h test. The test may be used although there was no analytical monitoring since the compound is not likely to be degraded by photolysis or hydrolysis and low volatility is given (Bayer AG 1985).

Description of key information

3 -chlor-p-toluidine was measured to cause LD50 = 24.8 mg/L using Danio rerio in a 96 h test. The test may be used although there was no analytical monitoring since the compound is not likely to be degraded by photolysis or hydrolysis and low volatility is given (Bayer AG 1985).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

24.8 mg/L