3-chloro-p-toluidine

Endpoint summary

Description of key information

Short-term toxicity to fish
3 -chlor-p-toluidine was measured to cause LD50 = 24.8 mg/L using Danio rerio in a 96 h test. The test may be used although there was no analytical monitoring since the compound is not likely to be degraded by photolysis or hydrolysis and low volatility is given (Bayer, 1985).

Short-term toxicity to aquatic invertebrates
A study was performed to assess the acute toxicity of 2-Chlor-4-toluidin to Daphnia magna STRAUS under static conditions. The study was conducted in accordance with the Council Regulation (EC) No 440/2008, Method C.2 ‘Acute toxicity for Daphnia’ (2008) which is equivalent to OECD Guideline for Testing of Chemicals No. 202 'Daphnia sp., Acute Immobilisation Test' (adopted April 13, 2004). The Daphnia were exposed to a range of concentrations of nominally 0.10, 0.22, 0.48, 1.1, 2.3, 5.2 and 11 mg/L of 2-Chlor-4-toluidin dissolved in dilution water. Auxiliary used to prepare the test media was a magnetic stirrer. Observations were made on the swimming ability and the percentage immobilised, respectively, after 24 and 48 hours of exposure. The following values were determined:
EC50 (24 h) = 1.5 mg/L
EC50 (48 h) ≥ 0.22 < 0.48
The results are expressed in terms of nominal concentrations. Measured test item exposure concentrations ranged from 90.6 % to 95.0 % of their nominal target values at 0 hours and from 86.3 % to 92.2 % of their nominal target values at 48 hours (Currenta, 2022).

Toxicity to aquatic algae
A study was performed to assess the adverse effects of 2-Chlor-4-toluidin on the growth rate (= rate of increase in cell density with time) and the yield (= biomass at time t minus initial biomass) of the planktonic freshwater algal species Desmodesmus subspicatus (former name: Scenedesmus subspicatus) over several generations. The study was conducted in accordance with Commission Regulation (EC) No 761/2009 amending Regulation No 440/2008, Method C.3 ‘Freshwater Alga and Cyanobacteria, Growth inhibition test’ (2009) which is equivalent to OECD Guideline for Testing of Chemicals No. 201 (2006). Exponentially growing algal cells were exposed for a period of 72 hours to a ange of concentrations, nominally 0.010, 0.032, 0.10, 0.32, 1.0, 3.2, 10, 32 and 100 mg/L of 2-Chlor-4-toluidin dissolved in dilution water. Auxiliary used to prepare the test media was a magnetic stirrer. The cell densities were measured at 24 hour intervals. Inhibition of the algal population was measured as reduction in growth rate (index r) and yield (y), relative to control cultures grown under identical conditions. The following values were determined:
ErC 50 (0-72 h): 15
ErC 10 (0-72 h): 6.1
The results are expressed in terms of nominal concentrations. Measured test item concentrations ranged from 98.2 % to 104.7 % of their nominal target values at 0 hours, and from 94.3 % to 99.8 % of nominal target values at 72 hours (Currenta, 2022).