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Ecotoxicological information

Short-term toxicity to fish

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short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
according to guideline
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
Details on sampling:
- Concentrations: 38 mg/l
- Sampling method:Representative water samples of exposure medium, control and solvent control was collected during the conduct of the experiment at
0 and 24h and sent to the department of analytical chemistry for the verification of concentration and stability.

- Sample storage conditions before analysis: no data
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: the final solution was warmed between 50-60°c and kept on a magnetic stirrer for 30 minutes to obtain clear solution. Then it was allowed to cool at room temperature and the same was transferred to the exposure medium.
- Eluate:
- Differential loading:
- Controls: control group (without test substance) and solvent control
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone as organic solvent
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): the solvent used in the exposure medium did not exceed 0.1 ml/l
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
- Common name: Brachydanio Rerio
- Strain:
- Source: supplied from a commercial fish farm.
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): 3 +- 0.5 cm
- Weight at study initiation (mean and range, SD):
- Method of breeding: /
- Feeding during test
- Food type: commercially available aquarium fish feed
- Amount:
- Frequency: daily

- Acclimation period:7 days
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: commercially available aquarium fish feed
- Feeding frequency: feeding was stopped 24h prior to the commencement of the experiment
- Health during acclimation (any mortality observed): healthy

QUARANTINE (wild caught)
no wild caught
- Duration:
- Health/mortality:
Test type:
Water media type:
Limit test:
Total exposure duration:
96 h
Post exposure observation period:
The fish was quarantined for a min. of at least 12d in the laboratory before they are used for testing and the fish was fed with
commercially available aquarium fish feed, daily.
Body weight was recorded and length measured in randomly selected ten fish prior to the commencement of the acclimatization.
The fish was acclimatized 7 days prior to the experiment in the test room and the feeding was stopped 24h prior to the commencement of the
control: 200 CaCO3 mg/l
Solvent control (acetone not exceeding 0.1 ml/l): 200 CaCO3 mg/l
38 mg/l: 20/04/2006: 184 mg/l CaCO3 -- 24/04/2006: 194 mg/l CaCO3.
Test temperature:
control: ca. 22.5°c
Solvent control (acetone not exceeding 0.1 ml/l): ca. 22.5°c
38 mg/l: ca. 22.5 - 25.4 °c
control: 8.21-8.26
Solvent control (acetone not exceeding 0.1 ml/l): 8.20 - 8.28
38 mg/l: 8.20 - 8.38
Dissolved oxygen:
control: 7.07 - 7.22 mg/l
Solvent control (acetone not exceeding 0.1 ml/l): 7.17 - 7.31 mg/l
38 mg/l: 7.19 - 7.28 mg/l
Nominal and measured concentrations:
38, 45.6, 54.7, 65.7 and 78.8 mg/L
Details on test conditions:
- Test vessel:glass aquaria (10l)
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: 10l
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):renewed daily
- No. of organisms per vessel:10
- No. of vessels per concentration (replicates):5
- No. of vessels per control (replicates):1
- No. of vessels per vehicle control (replicates):1
- Biomass loading rate:

- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:1120-1158µS
- Culture medium different from test medium:
- Intervals of water quality measurement:

- Adjustment of pH:8.20-8.38
- Photoperiod:
- Light intensity:12h light, 12h darkness

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:
Reference substance (positive control):
96 h
Dose descriptor:
Effect conc.:
> 38 mg/L
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Key result
96 h
Dose descriptor:
Effect conc.:
36.7 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
- Behavioural abnormalities: no signs of toxicity or behavioural abnormalities were observed in fish exposed to control and 38 mg/l BBSA.
- Observations on body length and weight:
- Other biological observations:
- Mortality of control: no mortality was observed in control group, solvent control group and fish exposed to 38 mg/l
- Other adverse effects control:
- Abnormal responses:
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:/
- Effect concentrations exceeding solubility of substance in test medium:/
Reported statistics and error estimates:
Validity criteria fulfilled:
Short-term toxicity to fish was assessed in a 96h study. The measured LC 50 > 38 mg/L (nominal), the highest concentration tested.

Description of key information

The acute fish toxicity of BBSA was determined in an OECD 203 test. The measured LC50 was 36.7 mg/l.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
36.7 mg/L

Additional information