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Short-term toxicity to fish

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Description of key information

The LC50 of 3.7 mg/L is taken as the key value for the CSA because it is the only valid study which has a defined result.
A study was performed to determine the acute toxicity of the test material DPGDB to fathead minnows (HLS 2000, VCL332/994962). The study was conducted according to EC, OECD, and US EPA test guidelines, and in compliance with GLP. The 96 h LC50 value for DPGDB with fathead minnow was 3.7 mg/L. The "no-observed effect concentration" (NOEC) for DPGDB with fathead minnow was 1.2 mg/L.

Key value for chemical safety assessment

LC50 for freshwater fish:
3.7 mg/L

Additional information

Studies were conducted to determine the acute toxicity of the test material DPGDB to freshwater fish (Rainbow Trout and fathead minnows). Studies with both species were conducted with the substance introduced using a water accommodated fraction (WAF) and by flow through. The studies were conducted according to EC, OECD, and US EPA test guidelines, and in compliance with GLP. The WAF studies suffered from low concentrations of the test substance being measured in the initial WAF solution and subsequent losses of the test substance. In addition, the results from the WAF tests with the two species showed no toxicity with one species and high toxicity with the other which was unexplainable. Because of the various problems with the two WAF tests, they are believed to be unreliable and have been disregarded. The flow through studies were well conducted exhibiting constant exposure levels throughout the studies and the results from both species were similar and in line with QSAR estimations. The results from the flow through studies are being used for the hazard determination and are presented below.

The 96 h LC50 value for DPGDB with fathead minnow was 3.7 mg/L. The "no-observed effect concentration" (NOEC) for DPGDB with fathead minnow was 1.2 mg/L (HLS 2000, VCL332/994962).

The 96 h LC50 value for DPGDB with rainbow trout was >3.0 mg/L. The "no-observed effect concentration" (NOEC) for DPGDB with rainbow trout was 3.0 mg/L (HLS 2000, VCL333/002268).

These values are supported by the QSAR modelling (EPIsuite ECOSAR v1.00, 2009) with a 96-hour LC50 value of 3.94 mg/l.