N,N'-bis(3-aminopropyl)ethylenediamine

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

08 Oct. 1990 to 12 Oct. 1990

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions, e.g. test item concentration and stability not verified

Qualifier:

according to guideline

Guideline:

other: German Industrial Standard test Guideline DIN 38412, part 15

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The product was added to the test water without any pretreatment; subsequently the fish were placed into the aquaria

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Goldorfe
- Strain: Leuciscus idus (golden variety)
- Source: Fischzucht Paul Eggers, 2354 Hohenwestedt, Germany
- Length at study initiation: 7.0 cm (range: 6.5 - 7.8 cm)
- Weight at study initiation: 3.4 g (range: 2.5 - 5.1 g)
- Feeding during test: no
- Frequency: Withdrawal of food before exposure: 1 day


ACCLIMATION
- Acclimation conditions (same as test or not): oil-free aerated and charcoal filtered tap water, flow-through system
Water temperature: 19-22°C
Acclimation to test water and test temperature: 3 days
- Type and amount of food: Growing feed, F/B 50, SSNIEF Spezialdiaeten GmbH, Soest, Germany
- Feeding frequency: ad libitum.

HOUSING
- Housing water: charcoal filtered dechlorinated water in continuous flow-through
- Aeration: oilfree air
- Light regime: 16 : 8 hours light : dark
- Hardness: 2.5 mM
- Oxygen concentration: > 60 % of maximum saturation
- pH: about 8.0
- Water temperature: 19 - 22 °C
- Duration of housing: about 1.5 month
- Medical treatment: Twice with 0.05 mg/L Malachitegreen chloride and once with 10 mg/L tetracyclinhydrochlorid
- Feeding: Growing feed, F/B 50, SSNIEF Spezialdiaeten GmbH, Soest, Germany
- Amount of feed: ad libitum
- Mortality during the last 2 weeks of housing: 0.1%

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

Total hardness: 2.5 mM

Test temperature:

20 - 23 °C

pH:

7.4 - 11.3

Dissolved oxygen:

7.2 - 9.1 mg/L

Nominal and measured concentrations:

Nominal: Control, 100, 215, 464, and 1000 mg/L; additionally a neutralised sample of the test concentration of 1000 mg/L was tested in parallel

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): aquaria open
- Material, size, fill volume: glass, 30 x 22 x 24 cm, 10 L
- Aeration: aeration with oil-free air
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 3.4 g fish /L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, part 11
- Ca/Mg ratio: 4 : 1
- Intervals of water quality measurement: 1, 24, 48, 72, and 96 hours

OTHER TEST CONDITIONS
- Photoperiod: 16:8 hour light:dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, symptoms

TEST CONCENTRATIONS
- Spacing factor for test concentrations: ca. 2.2
- Justification for using less concentrations than requested by guideline: not applicable
- Range finding study
- Test concentrations: 10, 100, 1000 mg/L
- Results used to determine the conditions for the definitive study: 96-h LC50: >100 - <1000 mg/L

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 220 - < 460 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: based on not neutralised test solutions

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: based on not neutralised test solutions

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: based on not neutralised test solutions

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

460 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: based on not neutralised test solutions

Details on results:

- Behavioural abnormalities: apathy, narcotic-like state, gasping, tumbling, exopthalmus
- Mortality of control: 0%
- Effect concentrations exceeding solubility of substance in test medium: no
- In addition to the test solutions, the highest test concentration of 1000 mg/L was tested in parallel after neutralisation. The mortality was reduced and toxic effects showed with a delay (not neutralised: 100% after 1h; neutralised: 80% after 48 h and 90% after 72 h).

Sublethal observations / clinical signs:

Cumulative mortality

Nominal conc. (mg/L)	Number of fish	Mortality (n; cumulated) after
		1 h	4 h	24 h	48 h	72 h	96 h
0	10	0	0	0	0	0	0
100	10	0	0	0	0	0	0
215	10	0	0	2	2	2	2
464	10	0	3	10	10	10	10
1000	10	10	10	10	10	10	10
1000*	10	0	0	0	8	9	9

* = pH adjusted

Symptoms 

Nominal conc. (mg/L)	Symptoms after
	1 h	4 h	24 h	48 h	72 h	96 h
0
100
215			A	A
464	A	ANT
1000
1000*			ALT	ET	AE	AT

*: pH-adjusted; A: Apathy, N: Narcotic like state, T: Tumbling, L: Gasping, E: Exophthalmos

pH values

Nominal conc. (mg/L)	pH values after
	1 h	24 h	48 h	72 h	96 h
0	7.9	7.9	7.7	7.7	7.9
100	10.4	9.2	8.3	8.1	8.1
215	10.7	9.9	9.1	8.6	8.2
464	11.1	10.6
1000	11.3
1000*	7.5	7.4	7.4	7.5	7.5

*: pH-adjusted

 

Description of key information

With high probability acutely not harmful to fish.

Additional information

The toxicity of N,N'-bis(3 -aminopropyl)ethylenediamine (CAS 10563 -26 -5) was studied in a static test acording to national standards (DIN 38412, part 11). The test item concentrations were not analytically verified, but are assumed to be stable regarding the sufficient recovery (> 80%) in the chronic study with Daphnia magna (BASF SE, 2014). The 96 -h LC50 was determined to be > 200 and < 460 mg/L. In addition to the not pH-adjusted test solutions, the highest test concentration of 1000 mg/L was tested in parallel after neutralisation. The mortality was reduced and toxic effects showed with a delay (not neutralised: 100% after 1h; neutralised: 80% after 48 h and 90% after 72 h; BASF AG, 1991; report no. 10F0227/895367).