2-(2-aminoethoxy)ethanol

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well documented report whcih meets basic scientific principles.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report Date:

1990

Materials and methods

Test guideline

Qualifier:

according to

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

no

Type of assay:

bacterial reverse mutation assay

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): 2,2-Aminoethoxyethanol
- Analytical purity: 99.6 %
- Other: room temperature

Method

Target gene:

Histidin operon

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Metabolic activation system:

S9-mix from Aroclor 1254 treated male Sprague-Dawley rats.

Test concentrations with justification for top dose:

All tester strains: 0, 20, 100, 500, 2500 and 5000 ug/plate (standard plate test and preincubation test);
TA 98: 12.5, 25, 50 and 100 ug/plate (preincubation test)

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: water

Controls

Untreated negative controls:

yes

Remarks:

water control

Negative solvent / vehicle controls:

yes

Remarks:

sterility control

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: with S-9 mix: all strains 2-aminoanthracene; without S-9 mix: strains TA100, TA1535: N-methyl-N'-nitro-N-nitrosoguanidine; TA98: 4-nitro-o-phenylendiamine; TA1537: 9-aminoacridine

Details on test system and experimental conditions:

METHOD OF APPLICATION: preincubation

DURATION
- Preincubation period: 20 min
- Exposure duration: 48 h

NUMBER OF REPLICATIONS: 3


METHOD OF APPLICATION: standard plate test

DURATION
- Exposure duration: 48 h

NUMBER OF REPLICATIONS: 3

Evaluation criteria:

The test chemical is considered positive in this assay if the following criteria are met: A dose-related and reproducible increase in the number of revertant colonies, i.e. about doubling of the spontaneous mutation rate in at least one tester strain either without S-9 mix or after adding a metabolizing system.

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

in the preincubation test without S-9 mix depending on the strain at doses > 2500 µg/plate.

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Additional information on results:

TEST-SPECIFIC CONFOUNDING FACTORS
- Water solubility: Complete solubility of test substance in aqua dest.

Remarks on result:

other: other: Salmonella typhimurium TA1535, TA100, TA1537, TA98

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

Standart plate test:

Dose (µg/plate)

TA1535

TA100

TA1537

TA98

-S9

+S9

-S9

+S9

-S9

+S9

-S9

+S9

0

19±4

16±2

95±10

104±11

9±1

12±3

24±5

30±2

20

15±6

11±6

103±13

107±7

10±3

14±4

23±4

33±2

100

18±6

17±2

103±12

105±3

7±2

13±1

22±2

33±3

500

14±5

17±5

93±21

99±9

13±2

18±2

17±2

36±6

2500

16±6

20±1

102±4

92±10

9±3

14±2

20±4

37±4

5000

14±4

21±2

90±11

107±7

14±6

18±3

21±1

30±2

2AA

-

215±10

-

1029±22

-

180±17

-

1003±61

MNNG

1332±112

-

1373±90

-

-

-

-

-

AAC

-

-

-

-

354±56

-

-

-

NOPD

-

-

-

-

-

-

861±14

-

Mean ± SD

Preincubation-test:

Dose (µg/plate)

TA1535

TA100

TA1537

TA98

-S9

+S9

-S9

+S9

-S9

+S9

-S9

+S9

0

12±2

12±1

82±4

100±9

7±2

11±2

20±2

37±4

20

12±4

14±2

76±3

93±16

8±3

13±3

18±6

60±2

100

11±4

13±1

79±3

107±9

6±4

15±1

24±5

46±5

500

13±6

11±4

87±2

88±10

5±2

13±2

27±1

53±8

2500

16±3

14±2

61±2

86±5

7±3

10±3

21±9

49±8

5000

10±8

16±3

42±12

81±6

x

15±5

14±2

34±3

2AA

-

271±15

-

1415±74

-

113±26

-

1330±95

MNNG

1310±216

-

1335±88

-

-

-

-

-

AAC

-

-

-

-

426±96

-

-

-

NOPD

-

-

-

-

-

-

804±62

-

Mean ± SD

Preincubation-test:

Dose (µg/plate)

TA98

-S9

+S9

0

26±4

34±2

12.5

22±2

35±4

25

20±6

36±3

50

28±2

33±2

100

26±3

33±2

2AA

-

753±8

NOPD

844±33

-

Mean ± SD

X: reduced background growth

2-AA: 2-aminoanthracene;

MNNG; N-methyl-N-nitro-N-nitrosoguanidine

NOPD: 4-nitro-o-phenylendiamine

AAC: 9-aminoacridine chloride monohydrate

Under the conditions tested 2-(2-aminoethoxy)ethanol in non mutagenic in the bacterial AMES-test.

The positive controls gave the expected values.