1,2,4-triazole

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1976

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP Guideline study without detailed documentations

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1976

Report date:

1976

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Version / remarks:

reliability scoring was based on 2002 guideline

Deviations:

yes

Remarks:

Chemical characteristics of test substance and temperature, light cycle, and humidity not provided; grading scale of irritation not included and an incorrect number of animals were used

GLP compliance:

no

Remarks:

study pre-dates GLP requirements

Test material

Test material information

Constituent 1

Reference substance name:

1,2,4-triazole

EC Number:

206-022-9

EC Name:

1,2,4-triazole

Cas Number:

288-88-0

Molecular formula:

C2H3N3

IUPAC Name:

1H-1,2,4-triazole

Details on test material:

- Name of test material (as cited in study report): 1,2,4-triazole
- Analytical purity: reported as technically pure

Test animals

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: from the breeder Winkelmann (Borchen)
- Age at study initiation: no data
- Weight at study initiation: 3-4kg
- Fasting period before study: no data
- Housing: singly in standard rabbit cages
- Diet (e.g. ad libitum): Rabbit food 222 (Hoeing co.), ad libitum
- Water (e.g. ad libitum): not precised, ad libitum
- Acclimation period: no data

ENVIRONMENTAL CONDITIONS: no data

Test system

Type of coverage:

occlusive

Preparation of test site:

other: hairless skin on ears

Vehicle:

unchanged (no vehicle)

Controls:

not required

Amount / concentration applied:

Small cellulose patches (1.5 cm X 1.5 cm) with 500 mg of the test substance applied was attached to skin of the ears of rabbits with an adhesive dressing for 24 hours.

Duration of treatment / exposure:

24 hours of exposure

Observation period:

7 days

Number of animals:

2

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

mean

Time point:

24 h

Score:

0

Max. score:

4

Results 2

Irritation parameter:

edema score

Basis:

mean

Time point:

24 h

Score:

0

Max. score:

4

Irritant / corrosive response data:

A score of 0 for all time points, factors and animals.

Other effects:

No changes for up to 7 days after application.

Applicant's summary and conclusion

Interpretation of results:

not irritating

Conclusions:

In this study, mean erythema score and mean edema score are equal to 0 : 1,2,4-triazole is not a skin irritant.

Executive summary:

A non GLP Acute Dermal Irritation / Corrosion test comparable to OECD 404 guideline was carried out with 1,2,4 -triazole. The test substance was applied undiluted on a occlusive patch on hairless skin on ears. The test material induced no erythema and no edema on two animals.

Under the experimental conditions, the test substance is not irritant and is not classified according to regulation EC no.1272/2008 (CLP).