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Toxicological information

Acute Toxicity: other routes

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Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1923
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
significant methodological deficiencies
Cross-reference
Reason / purpose:
reference to same study

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1923
Report Date:
1923

Materials and methods

Principles of method if other than guideline:
other: acute toxicity testing - subcutaneous application
GLP compliance:
no
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Tributylamin

Test animals

Species:
rat
Strain:
other: white
Sex:
not specified

Administration / exposure

Route of administration:
subcutaneous
Vehicle:
not specified
Doses:
no data
No. of animals per sex per dose:
6
Control animals:
not specified

Results and discussion

Effect levelsopen allclose all
Sex:
not specified
Dose descriptor:
LDLo
Effect level:
ca. 310 - 390 mg/kg bw
Remarks on result:
other: mean: 350 mg/kg bw
Sex:
not specified
Dose descriptor:
LD100
Effect level:
780 mg/kg bw
Remarks on result:
other: within 6 min after application
Mortality:
see table remarks on results
Clinical signs:
restlessness, reflex excitability, increased pulse rate, increased  respiration during the excitant state, dyspnea, convulsions of the  medullary type at first, later spinal, followed by depression, slowing of the  pulse and respiration, marked cyanosis of mucosa and tongue, coma, and  death.
Similar symptoms were noted in rabbits.
Gross pathology:
Collapsed lungs, congested and cyanotic viscera, heart dilatation, still  active intestinal peristalsis, dark discoloration of the blood.

Any other information on results incl. tables

The dose is given as 0.4 to 0.5 ml/kg bw respective mean 0.45 ml/ kg bw (conversion to mg/kg bw was done using the density d= 0.78 g/ccm).

[mg/kg bw]  number of animals used  mortality [%]
1560 -1950  100 
1170  100 
390 -780  13  69 
234 -312 

Applicant's summary and conclusion

Conclusions:
The data presented in this study are insufficient and do not fit the requirements for a definite interpretation of the results.
Executive summary:

Testing acute toxicity in rats led to a minimal fatal dose (LDLo) in the range of 310 to 390 mg test substance (Tributylamin) per kg bw and a fatal dose (LD100) of 780 mg/kg bw after subcutaneous application (Hanzlik, 1923).