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Toxicological information

Specific investigations: other studies

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Administrative data

Endpoint:
specific investigations: other studies
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well-documented publication/study report which meets basic scientific principles
Cross-reference
Reason / purpose:
reference to same study

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1989
Report Date:
1989

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
Examination of respiratory response following normal inhalation or tracheal intubation of the test substance
Type of method:
in vivo
Endpoint addressed:
respiratory irritation

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test animals

Species:
mouse

Results and discussion

Applicant's summary and conclusion

Executive summary:

Tributylamine (30 to 95 ppm) and other alkylamines was examined for respiratory irritation in CF-1 mice (4 animals per dose), either after normal inhalation for sensory irritation, or with tracheal intubation for the detection of pulmonary irritation. Like in many similar studies performed by the author the test procedure followed an ASTM method.

Unlike other amines, tributylamine caused deaths upon inhalation. Mortality was complete in animals exposed for 25 minutes to concentrations of 110 ppm and above.Also, tributylamine gave rise to time-frequency curves which differed from those of the other amines which were also examined in this study. In normal mice a fast decrease of the respiratory rate was seen, and which was due to sensory irritation. This effect was maximum within the first 2 minutes, but disappeared. Later, a slower decrease was noted, and the respiratory pattern indicated that this was due to pulmonary irritation. Following exposure there was little recovery.

In cannulated mice a small increase of the respiratory rate was noted, and then the rate decreased due to pulmonary irritation. The recovery was almost complete in these animals.

As a result, no RD50-value could be obtained for tributylamine. The tRD50-value was 96 ppm. The results indicate that tributylamine causes primarily pulmonary irritation, in contrast to other alkylamines. The authors derived a TLV proposal from their data (TLV=0.2 x RD50; and TLV = 0.01 x tRD50) in the range of 1 to 2 ppm (Nielsen, 1989).

The study is considered to be valid and suitable for assessment.