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EC number: 253-256-2 | CAS number: 36888-99-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP compliant guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- - Origin: SAG, Göttingen
- Cultivation at test facility: fresh stocks were prepared every month on Z-Agar; light intensity amounted to 35 - 70 µE*m-2*s-1 for 24 h per day
- Culture medium: nutrient medium Z according to LÜTTGE et al. (1994) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 21 - 23 °C
- pH:
- 7.58 - 10.22
- Nominal and measured concentrations:
- - Concentrations tested (mg/L): control, 0.625, 1.25, 2.5, 5, 10 (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: sterile Erlenmeyer flasks, volume: 250 ml (nominal)
- Fill volume: 100 ml (nominal)
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
INOCULUM
- 3 d old preculture was used as inoculum
- Initial cell concentration of ca. 2 - 5E3 cells/mL
GROWTH MEDIUM
- Nutrient medium Z according to LÜTTGE et al. (1994)
OTHER TEST CONDITIONS
- Photoperiod: 24h/d light
- Light intensity: 60 - 120 µE*m-2*s-1
- Agitation: test flasks were placed on rotary shaker and oscillated at ca. 70 rpm. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate p.a. (Sigma)
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- 72 h EC10 >10 mg/L nominal test mat. growth rate
72 h EC20 >10 mg/L nominal test mat. growth rate - Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: due to the low water solubility of the substance a saturated solution according to guideline OECD 23 The test substance (200.4 mg) was added to 2 l test medium. The mixture was placed in an ultrasonic bath for 5 minutes then stirred vigorously for about 3 days at 20 + 2 °C, to generate a saturated test solution. Undissolved test substance was removed by filtration (pore with 0.2 μm). - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Source (laboratory, culture collection): cultured at testing facility. The culture was originally obtained from the collection of algal cultures of the University of Göttingen /Germany - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Test temperature:
- 21 – 22 °C
- pH:
- 8.0 - 8.4
- Nominal and measured concentrations:
- Nominal test substance concentration:
control, 100 mg/l - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks (250 ml)
- Fill volume: 100 ml
- Initial cells density: 5000
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: the test medium was prepared according to the test guideline
OTHER TEST CONDITIONS
- Photoperiod: continous illumination
- Light intensity and quality: Artificial light, type universal white (OSRAM L 25), Light intensity: Average 5168 lux (within ±15% variability) at a wave length of 400 - 700 nm
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: cell counting (Neubauer haemocytometer)
- Chlorophyll measurement: in vivo-flourescence at a wave length of 432 nm
TEST CONCENTRATIONS
- Results used to determine the conditions for the definitive study: Ffluorescence C50 > 100 mg/l - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Reported statistics and error estimates:
- Student’s t-test
Referenceopen allclose all
Description of key information
With high probability acutely not harmful to aquatic algae.
Key value for chemical safety assessment
Additional information
The effect of the substance on the growth rate of green algae was investigated in a guideline study according to OECD 201. After 72 hours of exposure a NOEC >= 100 mg/l was determined (BASF SE, 2008).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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