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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Guideline study, a solubiliser was used
Qualifier:
according to guideline
Guideline:
OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)
GLP compliance:
no
Vehicle:
yes
Details on preparation of test solutions, spiked fish food or sediment:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: a stock solution was prepared by mixing 9.2 % test substance with about 20 % HC0 40 and about 70% water.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Hardened castor oil = HCO 40
Test organisms (species):
Cyprinus carpio
Details on test organisms:
TEST ORGANISM
- Common name: common carp
- Source: bred at testing facility. Teichwirtschaftlicher Beispielbetrieb der Bezirksregierung Oberpfalz, Wöllershof, Germany
- Weight at study initiation (mean): 36.3 g


ACCLIMATION
- Type and amount of food: Fukosaim Anschlussfutter 2, Fuko-Kraftfutterfabrik, Ulm, Germany
Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
8 wk
Test temperature:
25 ± 2 °C
Dissolved oxygen:
6.8 - 8.3 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: Giass vessel (130 l)
- Fill volume: 100 l
- Renewal rate of test solution (frequency/flow rate): 30 l/h per vessel; 720 l per day
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 1
- No. of vessels per control / vehicle control (replicates): 1


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water


Nominal and measured concentrations:
Nominal test substance concentrations:
1 mg/l and 0.1 mg/l
Analytically determined test concentrations [ppm]:
Nominal 1 mg/l: 0.966 (week 2), 0.987 (week 4), 0.966 (week 6), 0.956 (week 8)
Nominal 0.1 mg/l: 0.075 (week 2), 0.076 (week 4), 0.074 (week 6), 0.071 (week 8)
Type:
BCF
Value:
6.3
Remarks on result:
other: mean of two concentration factors measured after 8 weeks of exposure
Remarks:
Conc.in environment / dose:1 mg/l
Type:
BCF
Value:
10.8
Remarks on result:
other: mean of two concentration factors measured after 8 weeks of exposure
Remarks:
Conc.in environment / dose:0.1 mg/l
Details on results:
The acute toxicity was tested in a 48-hour TLm50 study conducted with Oryzias latipes: > 920 and < 1 380 mg active ingredient -dye/l.
No mortality in the bioaccumulation test observed.

Concentration factors in fish.

Nominal test substance concentration fish 2 weeks 4 weeks 6 weeks 8 weeks
1 mg/l a negative 4.14 1.7 10.15
b 0.42 2.24 3.41 2.44
0.1 mg/l a 11.40 20.00 21.90 1.20
b 19.20 negative 0.03 20.40
Endpoint:
bioaccumulation in aquatic species: fish
Data waiving:
study scientifically not necessary / other information available
Justification for data waiving:
other:
Reason / purpose for cross-reference:
data waiving: supporting information

Description of key information

Accumulation in organisms is not to be expected.

Key value for chemical safety assessment

Additional information

Accumulation in organisms is not to be expected.