Registration Dossier

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Fish

LC50 (96 h, freshwater) = 0.5 - 1 mg/L

Microorganisms

EC50 (activated sludge; 3 h) = > 10 mg/L - 100 mg/L

Additional information

Summary of aquatic toxicity

Fish

LC50 (96 h, freshwater) = 0.5 - 1 mg/L

The acute LC50 value for fresh water fish lies in a range between 0.5 and 1.0 mg/L. Five concentrations (0.22, 0.5, 1.0, 2.2 mg/L and a negative control with 0 mg/L) have been investigated in an acute toxicity test on zebra fish (Brachydanio rerio) according to OECD 203 and GLP over 96 hours in a static system. At the highest concentration a lethality of 100% was observed at the first inspection date.

Microorganisms

EC50 (activated sludge; 3 h) = > 10 mg/L - 100 mg/L

The effect of n/i-C13/C15 amine on the respiration rate of activated sludge was assessed in a GLP study according to OECD 209.

Samples of activated sludge were exposed to the test substance at nominal concentrations of 6.3, 12.5, 25.0, 50.0 and 100.0 mg/L for 3 hours. Their rates of oxygen consumption were determined and compared with those of controles, which were established at the beginning and end of the culture series.

The reference inhibitor 3,5 -dichlorophenol (3,5 -DCP) was employed in the test at 5, 20 and 30 mg/L, as a positive control. The three-hour 50% effect concentration (EC50) for 3,5 -DCP was calculated to be 12 mg/L (95% confidence limits 8 - 15 mg/L). These results show that the rest was valid and that the sample of activated sludge employed was sentitve to inhibition.

n/i-C13/C15 amine had an inhibitory effect on the respiration rate of activated sludge, resulting in an EC 50 value of > 10 mg/L - 100 mg/L.