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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
22 May 2007 to 26 May 2007
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
This study was conducted in accordance with international guidelines in a GLP testing facility.
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
yes
Remarks:
Modified to marine conditions (OSPAR/PARCOM)
Qualifier:
according to
Guideline:
other: OSPAR / PARCOM Protocols on methods for the testing of chemicals used in the offshore industry 2006
Deviations:
no
GLP compliance:
yes
Remarks:
GLP compliance statement only
Analytical monitoring:
no
Details on sampling:
N/A
Details on test solutions:
The behaviour of the test item in seawater was examined according to the method described in the Harmonised Offshore Chemical Notification Format (OSPAR 1995). A nominal 1000 mg/l solution was prepared in dilution water, shaken vigorously and allowed to stand for four hours.A clear colourless solution was obtained. For each nominal concentration the required amount of homogenised sample was added to 10 litres of dilution water.
Test organisms (species):
other: Scophthalmus maximus
Details on test organisms:
TEST ORGANISMTest Species: Turbot (Scopthalmus maximus)Source: Manx Mariculture, Derbyhaven, Castletown, Isle of Man IM9 1TUDate of Arrival at Laboratory: 07 Febraury 2007Number Received: 150Mortality in Stock after 48 hour Settling Period: 0 % during acclimation periodAccclimation Period: 07 February 2007 to 22 May 2007Acclimation Conditions: Temperature: 13.5 to 14.5 oCDissolved Oxygen: >92 % ASVPhotoperiod: 16 hours light and 8 hours darkFeeding: The Turbot were fed daily using Le Gouessant Turbot Label Rouge at a rate of approximately 2 % body weight per day until 24 hours before test initiation. The Turbot are not fed for the duration of the study.At the end of the test a sample of 10 turbot were weighed and measured.Mean length: 56.2 mmMean weight: 2.41 g
Test type:
semi-static
Water media type:
saltwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
N/A
Test temperature:
Refer to Table 1.
pH:
Refer to Table 1.
Dissolved oxygen:
Refer to Table 1.
Salinity:
Refer to Table 1.
Nominal and measured concentrations:
Nominal Concentrations: 1.908 mg/L (4.5 mg/L Amieton M33) with an untreated control (limit test)No analytical monitoring
Details on test conditions:
TEST SYSTEMTest Volume: 10 litres.Test Vessel: 12 litre volume aquaria.Number of replicates: 1 replicate at each control and test concentration.Test Organisms: 7 turbot were placed in each of the control and test vessels.Replacement Regime: Semi-static, test solutions replaced after 48 hours, the remaining animals being transferred to freshly prepared test solutions.Test Conditions: The test vessels were maintained at 15.0 ± 2.0oCWater Quality Measurements: The pH (to 0.1), dissolved oxygen (to 1 % ASV), temperature (to 0.5oC) and salinity (‰) were measured. Water quality determinations for each test and control solution were carried out immediately prior to initiating the test, after 24 hours, before and after the 48 hour change of test solutions, after 72 hours and at the end of the 96 hour exposure period.Observation Frequency: Observations and records of mortalities were made after 24, 48, 72 and 96 hours.Calculation and Expression of Results: Cumulative mortalities were calculated for each test concentration and control.
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
> 1.908 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: N/A
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 1.908 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: N/A
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
> 1.908 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: N/A
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 1.908 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: N/A
Details on results:
No abnormal behaviour was noted during the study.
Results with reference substance (positive control):
N/A
Reported statistics and error estimates:
No mortalities were observed in the control or test treatments, therefore no statistical analysis was necessary.

Table 1. Water Quality Parameters during the Study

a) Summary of pH values

Exposure period (hours)

Concentration (mg/L)

Control

1.908

0

8.2

8.3

24

8.2

8.2

48

8.2

8.2

8.2

8.3

72

8.3

8.2

96

8.2

8.2

pH of stock tank at test initiation: 8.2

b) Summary of dissolved oxygen values (% ASV)

Exposure period (hours)

Concentration (mg/L)

Control

1.908

0

97

97

24

99

98

48

99

97

96

97

72

99

98

96

97

96

Dissolved oxygen of stock tank at test initiation: 99 % ASV

c) Summary of temperature values (oC)

Exposure period (hours)

Concentration (mg/L)

Control

1.908

0

13.5

13.5

24

14.0

14.0

48

14.0

14.0

13.5

13.5

72

14.0

14.0

96

14.0

14.0

Temperature of stock tank at test initiation: 14.0oC

d) Summary of salinity (‰)

Exposure period (hours)

Concentration (mg/L)

Control

1.908

0

33

33

24

33

33

48

33

33

33

33

72

33

33

96

34

34

Salinity of stock tank at test initiation: 36 ‰

Table 2. Cumulative % Mortalities

Exposure period (hours)

Concentration (mg/L)

Control

1.908

0

0

0

24

0

0

48

0

0

72

0

0

96

0

0

Validity criteria fulfilled:
yes
Conclusions:
The 96 hour LC50 value of hexahydro-1,3,5-trimethyl-1,3,5-triazine to Turbot was estimated to be greater than 1.908 mg/L. The highest no observed effect concentration (NOEC) after 96 hours was 1.908 mg/L.
Executive summary:

Substance: 42.4 % hexahydro-1,3,5 -trimethyl-1,3,5 -triazine in water

Chemex Reference: Sample: ECO020727; Study: ENV8044

Test Species: Turbot (Scophthalmus maximus)

Test Type: Acute toxicity: 96 hour LC50

Test Conditions: OSPAR/PARCOM Protocols on Methods for the Testing of Chemicals used in the Offshore Industry 2006.

OECD 203: Fish Acute Toxicity Test.

Test Period: 22 May 2007 to 26 May 2007

Testing Laboratory: Chemex Environmental International Limited, Unit J, Broad Lane Industrial Estate, Cottenham, Cambridge CB24 8SW, UK.

Results:

Period of Exposure (hours)

LC50 (mg/L hexahydr-1,3,5 -trimethyl-1,3,5 -triazine)

24

> 1.908

48

> 1.908

72

> 1.908

96

> 1.908

 

96 hour NOEC

1.908 mg/L

96 hour 100 % mortality

Not determined

Description of key information

96 h LC50 (S. maximus) = >1.908 mg/L, OSPAR/PARCOM, Sacker (2007)

Key value for chemical safety assessment

LC50 for marine water fish:
1.908 mg/L

Additional information

A single key study was submitted for this endpoint, conducted in accordance with PARCOM guidelines. The 96h LC50 to S. maximus was estimated to be >1.908 mg/L (Sacker, 2007).