2-chlorobenzaldehyde

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

06 December 1984 to 21 December 1984

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: well performed study according to existing guidelines

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1985

Report date:

1985

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method B.1 (Acute Toxicity (Oral))

GLP compliance:

yes

Remarks:

according to OECD principles

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-chlorobenzaldehyde

EC Number:

201-956-3

EC Name:

2-chlorobenzaldehyde

Cas Number:

89-98-5

Molecular formula:

C7H5ClO

IUPAC Name:

2-chlorobenzaldehyde

Details on test material:

IUCLID4 Test substance: as prescribed by 1.1 - 1.4
- Name of test material (as cited in study report): o-Chlorbenzaldehyd D

- Storage condition of test material: in the dark, in a fume hood, 22°C

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: HOECHST AG, Kastengrund, SPF
- Age at study initiation: no data
- Weight at study initiation: males: 162 g - 190 g (average 177 g); females: 161 g - 193 g (average 173 g)
- Fasting period before study: about 16 hours
- Housing: 5 animals per makrolon cage
- Diet: Altromin 1324 rat diet, ad libitum
- Water (e.g. ad libitum): tap water, ad libitum
- Acclimation period: at least 5 days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 3°C
- Humidity (%): 50 +/- 20%
- Air changes (per hr): no data
- Photoperiod: 12 hrs dark / 12 hrs light

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: Sesame oil

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 25%

MAXIMUM DOSE VOLUME APPLIED: 5, 8, 12.6, 20 ml/kg bw

Doses:

1250, 2000, 3150, 5000 mg/kg bw

No. of animals per sex per dose:

5 animals per sex per dose

Control animals:

yes

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of weighing: weekly
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, gross pathology, histopathology

Results and discussion

Effect levels

Effect levels 1

Sex:

female

Dose descriptor:

LD50

Effect level:

ca. 3 150 mg/kg bw

Effect levels 2

Sex:

male

Dose descriptor:

LD50

Effect level:

>= 3 150 - <= 5 000 mg/kg bw

Mortality:

see below

Clinical signs:

other: Decreased spontaneous activity, stupor, flanks pinched in, ataxic gait,  squatting posture,  prone position, ruffled fur, palpebral fissure narrow, panting,  decreased respiration rate, mucilaginous feces. Clinical signs were reversible four days after ap

Gross pathology:

gross pathology of the animals which died:
stomach plump filled with food, yellowish to yellowish-green jelly mass in the small intestine, dark coloured adrenals, bladder plump filled, lung plethora;
no macroscopic findings in the animals which survived till the end of the study

Any other information on results incl. tables

mortality:

	mortality
dose	males		females
mg/kg bw	absolut	relative (%)	absolut	relative (%)
1250	0/5	0	0/5	0
2000	0/5	0	0/5	0
3150	1/5	20	2/5	40
5000	5/5	100	5/5	100

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information concluded by submitter Criteria used for interpretation of results: EU

Conclusions:

LD50 values for acute oral toxicity in male and female Wistar rats are between 3150 and 5000 mg/kg bw.

Executive summary:

Acute oral toxicity of 2-chlorobenzaldehyde has been investigated in male and female rats (5 animals per sex per dose). LD50 values for male rats were between 3150 and 5000 mg/kg bw and about 3150 mg/kg bw for female rats.

This well performed guideline study, which was in accordance with GLP principles has been judged to be reliable without restrictions (RL1) and was selected as key study. The test item has not to be classified according to the criteria of the EU directive 83/467/EWG.