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EC number: 500-097-4 | CAS number: 37353-75-6 1 - 4.5 moles propoxylated
Concentration % w/v
Control – AOO
AOO = Acetone/olive oil (vehicle control)
HCA = Hexyl cinnamic aldheyde (positive control)
NB Group 1 was the preliminary phase
The skin sensitisation potential of the test substance was determined in accordance with the OECD Guideline for Testing of Chemicals 429 using the Local Lymph Node Assay (LLNA). Preliminary investigations were performed at 50% w/v to establish the highest concentration of test substance which did not lead to systemic toxicity or excessive local irritation. The study comprised three treated groups, each comprising four female mice receiving the test substance at concentrations of 10, 25 or 50% w/v. Similarly constituted groups received the vehicle (acetone:olive oil (4:1 v/v)) or positive control substance (25% v/v hexyl cinnamic aldehyde). The mice were treated by daily application of 25 μl of the appropriate concentration or control (vehicle or positive), to the dorsal surface of both ears for three consecutive days. The proliferative response of the lymph node cells (LNC) from the draining auricular lymph nodes was assessed five days following the initial application, by measurement of the incorporation of 3H-methyl Thymidine (3HTdR) by β-scintillation counting of LNC suspensions. The response was expressed as radioactive disintegrations per minute per lymph node (dpm/node) and as the ratio of 3HTdR incorporation into LNC of test nodes relative to that recorded for control nodes (test/control ratio, termed as Stimulation Index (SI)). The results of preliminary investigations indicated that 50% w/v would be a suitable high concentration for use on the main study. The SI obtained for 10, 25 and 50% w/v were 1.2, 1.6 and 2.3 respectively which indicates that the test substance did not show the potential to induce skin sensitization. The EC3 value was greater than 50% w/v. The SI for the positive control substance hexyl cinnamic aldehyde was 9.0, which demonstrates the validity of this study. The test substance is not regarded as a potential skin sensitizer.
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