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EC number: 212-836-5 | CAS number: 873-32-5
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
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- Long-term toxicity to aquatic invertebrates
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Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989-08-03 - 1989-10-11
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2-chlorobenzonitrile
- EC Number:
- 212-836-5
- EC Name:
- 2-chlorobenzonitrile
- Cas Number:
- 873-32-5
- Molecular formula:
- C7H4ClN
- IUPAC Name:
- 2-chlorobenzonitrile
- Reference substance name:
- unknown
- IUPAC Name:
- unknown
- Test material form:
- solid
Constituent 1
impurity 1
Method
- Target gene:
- histidine-gene
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- rat liver post-mitochondrial fraction (S-9)
- Test concentrations with justification for top dose:
- initial range-finder: 0, 8, 40, 200, 1000, 5000 µg/plate
main experiment: TA98: 0, 500, 1000, 1500, 2000, 2500 (without S9)
main experiment: all other strains: 0, 1000, 1500, 2000, 2500, 3000 (without S9)
main experiment: all strains: 0, 1000, 2000, 3000, 4000, 5000 (with S9)
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- other: 2-aminoanthracene
- Statistics:
- F-test
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- highest concentration, w/o S9
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- small increas of revertants w/o S9
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- RANGE-FINDING/SCREENING STUDIES:
Initial range-finder treatment of strain TA100 was carried out 8, 40, 200, 100 and 5000 µg/plate. The treatment resulted in complete toxicity at 5000 µg/plate in the absence of S9, and a reduction in revertant numbers on plates treated with S9. In the main study concentrations were reduced to 2500 µg/plate (TA 98) and 3000 µg/plate (all other strains) in the absence of S9.
Applicant's summary and conclusion
- Conclusions:
- 2-chlorobenzonitrile did not induce mutation in five strains of Samonella typhimurim, when tested up the cytotoxicity limit, both in the absence and presence of metabolic activation.
- Executive summary:
In a reverse gene mutation assay in bacteria, strains TA 1535, TA 1537, TA 1538, TA 98 and TA 100 of S. typhimurium were exposed to 2-chlorobenzonitrile dissolved in dimethyl sulphoxide (DMSO) at concentrations of up to 5000 µg/plate in the presence and absence of mammalian liver post-mitochondrial fraction (S-9) as metabolic activation. There was no evidence of induced mutant colonies over background.
This study is classified as acceptable. This study satisfies the requirement for Test Guideline OECD 471 for in vitro mutagenicity (bacterial reverse gene mutation).
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