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The 48-h LC50 for Leuciscus idus (Goldorfe) in an acute toxicity test that was carried out in conformity with DIN 38 412 DEV L15 (ref. 1) and GLP, was determined to be 55 mg/L.

Effects of 2-chlorobenzonitrile on fish were recorded at the two highest concentrations tested (56 mg/L and 100 mg/L). At these concentrations undissolved test material could be found on the surface of the tests solutions. Therefore, mortality could also have been induced by physical effects.

Besides, short-term toxicity values for fish and daphnids were derived using EPISuite v3.12 (US-EPA 2004). The 96-h LC50 for fish and 48-h EC50 for daphnids were calculated to be 66 mg/L and 72 mg/L, respectively.

 

The 6-h EC50 (basis for effect: biomass) of 2-chlorobenzonitrile for Chlorella vulgaris was determined to be 43 mg/L (photometric determination of wavelength at 750 nm).

The TT (toxicity threshold) value of 2-chlorobenzonitrile is 43 mg/L for Pseudomonas putida. The 18-h EC50 and NOEC for Pseudomonas putida were determined to be 112 mg/L and 31 mg/L, respectively.

The 48-h EC50 of 2-chlorobenzonitrile to Tetrahymena pyriformis was determined to be 71 mg/L in the TETRATOX-Test.

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