Registration Dossier
Registration Dossier
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EC number: 235-113-6 | CAS number: 12069-69-1
Toxicity to soil macroorganisms except arthropods
Administrative data
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions. Acceptable study for assessing of the acute toxicity of copper to soil dwelling organisms. Peer reviewed by WHO.
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Toxicity of metals to the earthworm Eisenia fetida
- Author:
- Neuhauser EF et al.
- Year:
- 1 985
- Bibliographic source:
- Biol Fert Soils 1: 149-152
- Reference Type:
- secondary source
- Title:
- Environmental Health Criteria (EHC) 200, Copper
- Author:
- WHO
- Year:
- 1 998
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: EEC 79/831, Rev.3
Test material
- Reference substance name:
- Copper dinitrate
- EC Number:
- 221-838-5
- EC Name:
- Copper dinitrate
- Cas Number:
- 3251-23-8
- IUPAC Name:
- copper(2+) dinitrate
- Details on test material:
- - Analytical purity: no data
Constituent 1
Test substrate
- Vehicle:
- yes
Test organisms
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Common name: Eisenia fetida
- Weight at test initiation (mean and range, SD): 300-500 mg
Study design
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 14 d
Test conditions
- Test temperature:
- 20°C
- pH:
- 6.0 ± 0.5
- Moisture:
- 35%
- Details on test conditions:
- TEST SYSTEM
- Test container (material, size): glas container, 6.5 h * 12 cm diamter
- Amount of soil or substrate: 400 mg dw
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Soil texture (artificial soil)
- % sand: 69
- % silt: 10
- % clay: 20
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality
VEHICLE CONTROL PERFORMED: yes
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 379 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- CH2Cu2O5
- Basis for effect:
- mortality
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 217.8 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- element
- Remarks:
- Cu
- Basis for effect:
- mortality
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
