Copper(II) carbonate-copper(II) hydroxide (1:1)

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions. Endpoint EC50 is not in accordance with today´s guideline demands.

Data source

Reference

Reference Type:

publication

Title:

Evaluation of the OECD activated sludge, respiration inhibition test

Author:

Klecka GM, Landi LP

Year:

1985

Bibliographic source:

Chemosphere 14(9): 1239-1251

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Version / remarks:

1981

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

7758-99-8

EC Number:

616-477-9

Cas Number:

7758-99-8

IUPAC Name:

7758-99-8

Constituent 2

Reference substance name:

Copper sulphate, pentahydrate

IUPAC Name:

Copper sulphate, pentahydrate

Details on test material:

- Name of test material (as cited in study report): CuSO4*5H2O
- Analytical purity: reagent grade

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Fresh stock solution has been prepared (< 2 days); concentration: 0.5 to 5.0 g/L; pH value after correction: 7.5

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

- Method of cultivation: Activated sludge from a local municipal wastewater treatment plant
- Preparation of inoculum for exposure: The inoculum was washed and aerated during the night. 200 ml were added to a total volume of 500 ml.
- Initial biomass concentration: 800 mg/L dw

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Test conditions

Test temperature:

21°C

pH:

7.4-8.0 during test, 7.5 after correction at the beginning

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass, BOD-flask, liquid volume: 500 ml
- Aeration: sparged with air

TEST MEDIUM / WATER PARAMETERS
- Culture medium: OECD test medium

OTHER TEST CONDITIONS
- Adjustment of pH: no pH adjustments during test

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
O2: on line measurement, 5 min

Reference substance (positive control):

yes

Remarks:

3.5-dichlorophenol (CAS 591-35-5)

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

15.1 mg/L

Nominal / measured:

nominal

Conc. based on:

element

Remarks:

Cu

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

26.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

CH2Cu2O5

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Reported statistics and error estimates:

CI = 38.2-53.7 mg/l