Copper(II) carbonate-copper(II) hydroxide (1:1)

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

other information

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study, most sensitive NOEC in EHC 200 for algae toxicity.

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Biotests using unicellular algae and ciliates for predicting long-term effects of toxicants

Author:

Schaefer H et al.

Year:

1994

Bibliographic source:

Ecotoxicol Environ Saf 27: 64-81

Reference 2

Reference Type:

secondary source

Title:

Environmental Health Criteria (EHC) 200, Copper

Author:

WHO

Year:

1998

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Version / remarks:

1984

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Copper sulphate, pentahydrate

IUPAC Name:

Copper sulphate, pentahydrate

Constituent 2

Reference substance name:

7758-99-8

EC Number:

616-477-9

Cas Number:

7758-99-8

IUPAC Name:

7758-99-8

Details on test material:

- Analytical purity: p.a.

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Chlamydomonas reinhardtii

Details on test organisms:

TEST ORGANISM
- Common name: Chlamydomonas reinhardtii
- Strain: 11-3Qa
- Source (laboratory, culture collection): SAG

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

20°C

pH:

6.2

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: glas, erlenmayer flasks, yes
- Aeration: no
- Initial cells density: 2*10E5 cells/ml
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3

GROWTH MEDIUM
- Standard medium used: yes, SAG medium 12

OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: 24h
- Light intensity and quality: 8000 lux, cool white fluorescent lamps


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: electronic particle counter

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

0.14 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Remarks:

CH2Cu2O5

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.009 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Remarks:

CH2Cu2O5

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

0.079 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

element

Remarks:

Cu

Basis for effect:

growth rate

Effect concentrations 4

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.005 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

element

Remarks:

Cu

Basis for effect:

growth rate

Any other information on results incl. tables

In addition a flow through test was performed, resulting in: NOEC (96h) = 0.026 mg/L Cu and EC50 (96h) = 0.047 mg/L Cu.