Dimethyl(octyl)amine

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

11/03/1996-15/03/1996

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Similar to the guideline 203 and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Dimethyl(octyl)amine

EC Number:

230-939-3

EC Name:

Dimethyl(octyl)amine

Cas Number:

7378-99-6

Molecular formula:

C10H23N

IUPAC Name:

dimethyl(octyl)amine

Details on test material:

- Physical state: a clear, colourless liquid
- Storage condition of test material: in the dark under ambient conditions until use.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: rainbow trout
- Source: Wilmintong trout Farm
- Length at study initiation (length definition, mean, range and SD): 4.8 cm
- Weight at study initiation (mean and range, SD): 1.2 g

Study design

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

220-246 mg/L CaCO3

Test temperature:

13.2-14.6

pH:

8.0-8.6

Dissolved oxygen:

75-107

Nominal and measured concentrations:

0.1, 0.32, 1, 3.2, 10, 32 and 100 mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

5.7 mg/L

Effect concentrations 2

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

0.1 mg/L

Any other information on results incl. tables

Sublethal observations / clinical signs:

Observation times (hours)	Nominal concnetrations, mg/L
	0	0,1	0,32	1	3,2	10	32	100
0,25	0	0	0	0	0	2	5	5
2	0	0	0	0	0	5	5	5
24	0	0	0	0	0	5	5	5
48	0	0	0	0	0	5	5	5
72	0	0	0	0	0	5	5	5
96	0	0	0	0	0	5	5	5

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The no-observed-effect concentration (NOEC) was 0.1 mg/L. The 96 hour median lethal concnetration (LC50) of the substance to the rainbow trout was 5.7 mg/L

Executive summary:

The acute lethal toxicity of the susbtance to the rainbow trout was assessed under static conditions over a period of 96h. Group of five fish were exposed to the substance at nominal concentrations of 0.1, 0.32, 1, 3.2, 10, 32 and 100 mg/L. After 96h, the highest concentration at wich no mortality had occured was 3.2 mg/L and the lowest at which there was 100% mortality was 10mg/L. The no-observed-effect concentration (NOEC) was 0.1 mg/L. The 96 hour median lethal concnetration (LC50) of the substance to the rainbow trout was 5.7 mg/L